ArtNr |
NKM-ATGP3431-20ug |
Hersteller |
NKMAX
|
Menge |
20ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
Konjugat/Tag |
His-Tag |
Purity |
> 90% by SDS - PAGE |
Sequence |
MGSSHHHHHH SSGLVPRGSH MTTSASSHLN KGIKQVYMSL PQGEKVQAMY IWIDGTGEGL RCKTRTLDSE PKCVEELPEW NFDGSSTLQS EGSNSDMYLV PAAMFRDPFR KDPNKLVLCE VFKYNRRPAE TNLRHTCKRI MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP YYCGVGADRA YGRDIVEAHY RACLYAGVKI AGTNAEVMPA QWEFQIGPCE |
NCBI |
NP_001028216 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Glutamine synthetase, GS, GLNS |
Lieferbar |
|
Activity |
Specific activity is >2, 800 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH. |
Antigen Species |
Human |
Applications info |
Enzyme Activity, SDS-PAGE |
Biological activity |
Specific activity is >2, 800 pmol/min/ug, and is defined as the amount of enzyme that convert 1.0 pmole of L-glutamate to L-glutamine per miunte at pH 7.5 at 37C in coupled system with PK/LDH. |
Concentration |
1 mg/ml (determined by Bradford assay) |
Description |
Glutamine synthetase (GLUL), which is therefore able to regulate intracellular concentrations of glutamate. GLUL catalyzes the synthesis of glutamine from glutamate and ammonia. Glutamine is a main source of energy and is involved in cell proliferation, inhibition of apoptosis, and cell signaling. GLUL is essential for proliferation of fetal skin fibroblasts and plays an important role in controlling body pH by removing ammonia from circulation. Mutations in GLUL are associated with congenital glutamine deficiency. Recombinant GLUL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
Domain |
1-373aa |
Expression System |
E.coli |
Formulation |
Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 5mM DTT, 200mM NaCl |
Gene Category |
Enzymes & Proteases, Neuroscience |
General References |
Vermeulen T., et al, (2008) Arch Biochem Biophys. 478:96-102 Liaw SH., et al. (1995) Protein Sci. 4 (11): 2358-65. |
Molecular Weight |
44.2kDa (393aa), confirmed by MALDI-TOF |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
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