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Recombinant human NME3 protein

ArtNr NKM-ATGP3260-20ug
Hersteller NKMAX
Menge 20ug
Kategorie
Typ Proteins Recombinant
Specific against other
Konjugat/Tag His-Tag
Purity > 95% by SDS - PAGE
Sequence MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE
NCBI NP_002504
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Nucleoside diphosphate kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3
Lieferbar
Activity
Specific activity is > 150 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
Antigen Species
Human
Applications info
Enzyme Activity, SDS-PAGE
Biological activity
Specific activity is > 150 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
Concentration
0.5 mg/ml (determined by Bradford assay)
Description
NME3, also known as, a potential suppressor of metastasis, is expressed at a much lower level in highly metastatic cells than in cells with lower metastatic potential. It is important for the synthesis of nucleoside triphosphates and may play a role in apoptosis induction and hematopoiesis. It is preferentially expressed during early stages of myeloid differentiation of highly purified CD34+ cells. Recombinant human NME3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Domain
22-169aa
Expression System
E.coli
Formulation
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 50% glycerol, 0.1M NaCl, 2mM DTT
Gene Category
Metabolism, Signal Transduction
General References
Martinez R, et al. (1997). Cancer Res. 57: 1180-1187.
Masse K., et al. (2002). Gene., 296: 87-97
Molecular Weight
19.1 kDa (169aa), confirmed by MALDI-TOF
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 20ug
Lieferbar: In stock
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