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Recombinant human NME2 protein

Recombinant human NME2 protein

ArtNr	NKM-ATGP3251-100ug
Hersteller	NKMAX
Menge	100ug
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Specific against	other
Konjugat/Tag	Non-Tagged
Purity	> 90% by SDS - PAGE
Sequence	MANLERTFIA IKPDGVQRGL VGEIIKRFEQ KGFRLVAMKF LRASEEHLKQ HYIDLKDRPF FPGLVKYMNS GPVVAMVWEG LNVVKTGRVM LGETNPADSK PGTIRGDFCI QVGRNIIHGS DSVKSAEKEI SLWFKPEELV DYKSCAHDWV YE
NCBI	NP_001018149
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Nucleoside diphosphate kinase B, NDPK-B, NDPKB, NM23-H2, NM23B
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Activity	Specific activity is > 1, 800 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
Antigen Species	Human
Applications info	Enzyme Activity, SDS-PAGE
Biological activity	Specific activity is > 1, 800 units/mg, and is defined as the amount of enzyme that convert 1.0 umole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25C in a couple system with PK/LDH.
Concentration	1 mg/ml (determined by Bradford assay)
Description	NME2, also known as NM23B, is a heterodimeric protein functioning as a nucleoside diphosphate (NDP) kinase. NME1 and NME2 comprise the 152 amino acid A and B polypeptide chains of the NM23 enzyme, respectively. NME2 is identical to the beta subunit of human erythrocyte NDP kinase. NDP kinases are involved in the synthesis of nucleoside triphosphates, and NM23 may act in the regulation of signal transduction by complexing with G proteins, causing activation/inactivation of developmental pathways. Recombinant human NME2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Domain	1-152aa
Expression System	E.coli
Formulation	Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
Gene Category	Metabolism, Signal Transduction, Cancer
General References	Munkonge FM., et al. (2009) J Biol Chem. 284(39):26978-87. Treharne KJ., et al. (2009) FEBS Lett. 583(17):2789-92.
Molecular Weight	17.2 kDa (152aa), confirmed by MALDI-TOF
Storage	Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

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Menge: 100ug

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Lieferung vsl. bis 04.10.2024

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