ArtNr |
NKM-ATGP1397-100ug |
Hersteller |
NKMAX
|
Menge |
100ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
Konjugat/Tag |
His-Tag |
Purity |
> 95% by SDS - PAGE |
Sequence |
MGSSHHHHHH SSGLVPRGSH MGSHMSGFST EERAAPFSLE YRVFLKNEKG QYISPFHDIP IYADKDVFHM VVEVPRWSNA KMEIATKDPL NPIKQDVKKG KLRYVANLFP YKGYIWNYGA IPQTWEDPGH NDKHTGCCGD NDPIDVCEIG SKVCARGEII GVKVLGILAM IDEGETDWKV IAINVDDPDA ANYNDINDVK RLKPGYLEAT VDWFRRYKVP DGKPENEFAF NA |
NCBI |
NP_066952 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Inorganic pyrophosphatase, IOPPP, PP, PP1, SID6-8061 |
Lieferbar |
|
Antigen Species |
Human |
Applications info |
SDS-PAGE |
Concentration |
0.5mg/ml (determined by Bradford assay) |
Description |
PPA1 (Pyrophosphatase) belongs to the PPase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. The hydrolysis of inorganic pyrophosphate (PPi) to two phosphate ions is utilized in many biochemical pathways to render reactions effectively irreversible. Inorganic pyrophosphatase catalyzes this hydrolysis reaction in the early steps of lipid degradation, a prominent example of this phenomenon. By promoting the rapid hydrolysis of pyrophosphate (PPi), Inorganic pyrophosphatase provides the driving force for the activation of fatty acids destined for oxidation. Recombinant human PPA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
Domain |
1-289aa |
Expression System |
E.coli |
Formulation |
Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl |
Gene Category |
Metabolism |
General References |
Carman GM, et al. (2006) Trends Biochem. Sci. 31 (12): 694-9. usui Y, et al. (2010) J. Dent. Res. 89 (5): 504-9. |
Molecular Weight |
35.2kDa (313aa), confirmed by MALDI-TOF |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
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