ArtNr |
NKM-ATGP0669-100ug |
Hersteller |
NKMAX
|
Menge |
100ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
Konjugat/Tag |
His-Tag |
Purity |
> 85% by SDS - PAGE |
Sequence |
MGSSHHHHHH SSGLVPRGSH MASSSGNDDD LTIPRAAINK MIKETLPNVR VANDARELVV NCCTEFIHLI SSEANEICNK SEKKTISPEH VIQALESLGF GSYISEVKEV LQECKTVALK RRKASSRLEN LGIPEEELLR QQQELFAKAR QQQAELAQQE WLQMQQAAQQ AQLAAASASA SNQAGSSQDE EDDDDI |
NCBI |
NP_001929 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Down-regulator of transcription 1 TBP-binding, NC2, NC2-BETA, Down-regulator of transcription 1, TBP-binding |
Lieferbar |
|
Antigen Species |
Human |
Applications info |
SDS-PAGE |
Concentration |
0.5 mg/ml (determined by Bradford assay) |
Description |
DR1, also known as NC2-beta, is a TBP (TATA box-binding protein) associated phosphoprotein that represses both basal and activated levels of transcription. The protein is phosphorylated in vivo and this phosphorylation affects its interaction with TBP. This protein contains a histone fold motif at the amino terminus, a TBP-binding domain, and a glutamine- and alanine-rich region. By selectively repressing polymerases II and III, DR1 may shift the physiological balance of transcriptional output in favor of polymerase I. Recombinant human DR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography |
Domain |
1-176aa |
Expression System |
E.coli |
Formulation |
Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 100 mM NaCl, 0.1 mM PMSF , 10% Glycerol |
Gene Category |
Epigenomics (Transcription & Translation) |
General References |
White RJ., et al. (2010) Nucleic Acids Res. 38(4):1228-39. Collart MA., et al. (2008) Nucleic Acids Res. 36(2):539-49. |
Molecular Weight |
21.6 kDa(196aa), confirmed by MALDI-TOF |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
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