ArtNr |
NKM-ATGP0600-100ug |
Hersteller |
NKMAX
|
Menge |
100ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
Konjugat/Tag |
His-Tag |
Purity |
> 95% by SDS - PAGE |
Sequence |
MGSSHHHHHH SSGLVPRGSH MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV LPVVKKVEQK IANDNSLNHE YLPILGLAEF RSCASRLALG DDSPALKEKR VGGVQSLGGT GALRIGADFL ARWYNGTNNK NTPVYVSSPT WENHNAVFSA AGFKDIRSYR YWDAEKRGLD LQGFLNDLEN APEFSIVVLH ACAHNPTGID PTPEQWKQIA SV |
NCBI |
NP_002070 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase 1, Transaminase A, GIG18, Glutamic-oxaloacetic transaminase 1, Aspartate aminotransferase cytoplasmic, GIG 18, GIG18, Glutamate oxaloacetate transaminase1, Glutamic oxaloacetic transaminase 1 soluble, GOT 1, Growth inhibiting protein 18, SGOT, Transaminase, |
Lieferbar |
|
Antigen Species |
Human |
Applications info |
SDS-PAGE |
Concentration |
0.5 mg/ml (determined by Bradford assay) |
Description |
Glutamic-oxaloacetic transaminase (GOT) is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Recombinant human GOT1, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. |
Domain |
1-413aa |
Expression System |
E.coli |
Formulation |
Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol, 2mM DTT, 100mM NaCl |
Gene Category |
Metabolism |
General References |
Bousquet-Lemercier B., et al. (1990) Biochemistry 29 (22): 5293-9. Totan A., et al. (2006) Clin Chem Lab Med. 44(5):612-5. |
Molecular Weight |
48.4kDa (433aa) confirmed by MALDI-TOF |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
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