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Recombinant human ERp57/PDIA3 protein

ArtNr NKM-ATGP0462-100ug
Hersteller NKMAX
Menge 100ug
Kategorie
Typ Proteins Recombinant
Specific against other
Konjugat/Tag His-Tag
Purity > 95% by SDS - PAGE
Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMSDV LELTDDNFES RISDTGSAGL MLVEFFAPWC GHCKRLAPEY EAAATRLKGI VPLAKVDCTA NTNTCNKYGV SGYPTLKIFR DGEEAGAYDG PRTADGIVSH LKKQAGPASV PLRTEEEFKK FISDKDASIV GFFDDSFSEA HSEFLKAASN LRDNYRFAHT NVESLVNEYD DNGEGIILFR PSHLTNKFED KT
NCBI NP_005304
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Protein disulfide-isomerase A3, ER60, ERp57, ERp60, ERp61, GRP57, GRP58, HsT17083, P58, PI-PLC, Protein disulfide-isomerase A3 58 kDa glucose regulated protein, 58 kDa microsomal protein, Disulfide isomerase ER 60, ER p57, ERp 57, Glucose Regulated Protein 58 Kd, GRP 57, GRP 58, HsT17083, PDIA 3, PDIA3, Phospholipase C alpha, PI PLC, Protein disulfide isomerase A3, Protein disulfide isomerase family A member 3.
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Antigen Species
Human
Applications info
SDS-PAGE
Concentration
1 mg/ml (determined by Bradford assay)
Description
PDIA3, also known as protein disulfide-isomerase A3, is a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. This protein has protein disulfide isomerase activity. PDIA3 is also part of the major histocompatibility complex (MHC) class I peptide-loading complex (TAP1), which is essential for formation of the final antigen conformation and export from the endoplasmic reticulum to the cell surface. Recombinant human PDIA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Domain
25-505aa
Expression System
E.coli
Formulation
Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 0.1 M NaCl, and 10% glycerol
Gene Category
Heat Shock Proteins
General References
Vigneron N., et al. (2009) Eur J Immunol. 39(9):2371-6.
Forster ML., et al. (2009) J Biol Chem. 284(19):13045-56.
Molecular Weight
58.5 kDa (518aa), confirmed by MALDI-TOF.
Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100ug
Lieferbar: In stock
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