ArtNr |
NKM-ATGP0404-0.5mg |
Hersteller |
NKMAX
|
Menge |
0.5mg |
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
Konjugat/Tag |
Non-Tagged |
Purity |
> 90% by SDS - PAGE |
Sequence |
MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IG |
NCBI |
NP_001419 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Non-Neuronal Enolase (NNE), Enolase-1, ENO1, MBP-1, MYC promoter-binding protein 1, PPH, tau-crystallin, 2 phospho D glycerate hydro lyase, Alpha enolase, C myc promoter binding protein, EC 4.2.1.11, ENO1L1, Enolase 1, Enolase 1 (alpha), Enolase 1 (alpha) like 1, MBP 1, MBP1, MBPB1, MPB 1, MPB1, PPH, MYC promoter binding protein 1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, |
Lieferbar |
|
Activity |
Specific activity is > 20, 000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C. |
Antigen Species |
Human |
Applications info |
Enzyme Activity, SDS-PAGE |
Biological activity |
Specific activity is > 20, 000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C. |
Concentration |
1 mg/ml (determined by Bradford assay) |
Description |
Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant human alpha-enolase was expressed in E. coli and purified by using conventional chromatography. |
Domain |
1-434aa |
Endotoxin |
< 1.0 EU per 1ug of protein (determined by LAL method) |
Expression System |
E.coli |
Formulation |
Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM MgSO4, 10% Glycerol |
Gene Category |
Metabolism |
General References |
Das R., et al. (2009) Blood. 113(22):5371-2. ueno NT., et al. (2008) Cancer Res. 68(22):9302-10. |
Molecular Weight |
47.1 kDa (434aa), confirmed by MALDI-TOF. |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
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