Recombinant human Enolase 1/Non-neural enolase protein

Human

Specific activity is > 20, 000pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37C.

Alpha-enolase, also known as Enolase 1, is one of three enolase isoenzymes and a glycolytic enzyme expressed in most tissues. This protein plays a key role in anaerobic metabolism under hypoxic conditions and may act as a cell surface plasminogen receptor during tissue invasion. Abnormal expression of alpha-enolase is associated with tumor progression in some cases of breast and lung cancer. It also has been identified as an autoantigen associated with Hashimoto's encephalopathy and severe asthma. Recombinant human alpha-enolase was expressed in E. coli and purified by using conventional chromatography.

< 1.0 EU per 1ug of protein (determined by LAL method)

Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM MgSO4, 10% Glycerol

Das R., et al. (2009) Blood. 113(22):5371-2.
ueno NT., et al. (2008) Cancer Res. 68(22):9302-10.

Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.