ArtNr |
NKM-ATGP0281-100ug |
Hersteller |
NKMAX
|
Menge |
100ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Specific against |
other |
Konjugat/Tag |
Non-Tagged |
Purity |
> 95% by SDS - PAGE |
Sequence |
MEELPAPVKA IEKQGITIIK TFDAPGGMKG YLGKYQDMGV TIYLTPDGKH AISGYMYNEK GENLSNTLIE KEIYAPAGRE MWQRMEQSHW LLDGKKDAPV IVYVFADPFC PYCKQFWQQA RPWVDSGKVQ LRTLLVGVIK PESPATAAAI LASKDPAKTW QQYEASGGKL KLNVPANVST EQMKVLSDNE KLMDDLGANV TPAIYYMSKE NTLQQAVGLP DQKTLNIIMG NK |
NCBI |
NP_415137 |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Thiol:disulfide interchange protein dsbG, ybdP, Thiol:disulfide interchange protein dsbG ECK0598, JW0597, Periplasmic disulfide isomerase/thiol disulphide oxidase, |
Lieferbar |
|
Antigen Species |
E.coli |
Applications info |
SDS-PAGE |
Concentration |
1 mg/ml (determined by Bradford assay) |
Description |
Dsb proteins control the formation and rearrangement of disulfide bonds during the folding of secreted and membrane proteins in bacteria. DsbG, a member of this family, has disulfide bond isomerase and chaperone activity. So DsbG was shown to interact with refolding intermediates of chemically denatured citrate synthase and prevents their aggregation in vitro. In addition to sharing sequence homology with the thiol disulfide exchange protein DsbC, DsbG likewise was shown to form a stable periplasmic dimer and it displays an equilibrium constant with glutathione comparable with DsbA and DsbC. DsbG was found to be expressed at approximately 25% the level of DsbC. Recombinant DsbG was expressed in E. coli and purified by conventional chromatography techniques. |
Domain |
18-248aa |
Expression System |
E.coli |
Formulation |
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 2 mM EDTA, 10% glycerol |
Gene Category |
Heat Shock Proteins |
General References |
Riley M., et al (2006) Nucleic Acids Res. 34(1):1-9. Andersen CL., et al (1997) Mol Microbiol. 26(1):121-32 |
Molecular Weight |
25.8 kDa(232 aa), confirmed by MALDI-TOF. |
Storage |
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
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