MLS000544460

352336-36-8

341.36

10 mM in DMSO

Metabolic Enzyme/Protease

MLS000544460 is a highly selective and reversible Eya2 phosphatase inhibitor with a K_d of 2.0 uM and an IC₅₀ of 4 uM. MLS000544460 inhibit Eya2 phosphatase mediated cell migration and has anti-cancer activity^[1]. IC50 & Target: Kd: 2.0 uM (Eya2 phosphatase)^[1]
IC50: 4 uM (Eya2 phosphatase)^[1] In Vitro: MLS000544460 does not bind Eya3 phosphatase (ED)^[1].
MLS000544460 (10 uM) inhibits Eya2-mediated cell migration in human Eya2, Eya2 phosphatase-dead (D274N), and YFP (as a negative control) expressing MCF10A cells^[1].
When Eya2 ED is dialyzed into 10 uM EDTA to remove Mg²⁺, it binds MLS000544460 with a higher affinity (K_d=0.80 uM), indicating that this class of compounds does not require Mg²⁺ for its interaction with the Eya2 ED^[1].