M-31850

281224-40-6

463.48

10 mM in DMSO

Others

M-31850 is a potent, selective and competitive beta-hexosaminidase (Hex) inhibitor with IC50s of 6.0 uM and 3.1 uM for human HexA and human HexB, respectively. M-31850 also competitively inhibits beta-N-acetyl-D-hexosaminidase OfHex2 with a Ki of 2.5 uM.
IC50 & Target: IC50: 6.0 uM (human HexA) and 3.1 uM (human HexB).

Ki: 2.5 uM (OfHex2)
In Vitro: M-31850 shows some activity towards Jack Bean Hex (JBHex) and bacterial Hex from Streptomyces plicatus (SpHex) (IC50 of 280 uM and >500 uM for JBHex and SpHex, respectively).

M-31850 produces a dose dependent increase in MUG hydrolysis (Hex S levels) in lysates from treated infantile Sandhoff disease (ISD) cells.

M-31850 increases the half-life of the mutant Hex A from Adult forms of Tay-Sachs (ATSD) cells more than two-fold at 44 C, relative to the enzyme heated in the presence of DMSO. M-31850 acts as a classic competitive inhibitor of Hex (Km increases and Vmax is unaffected by increasing amounts of M-31850), with a Ki of 0.8 uM.