SAPAP Detection Set

These polyclonal antibodies can be used for detection of SAPAP1 - 4 by immunoblot at 0.5 - 2 μ g/mL, and for detection of SAPAP1 - 3 by immunohistochemistry at 2.5 - 5 μ g/mL, and Immunoflourescence.

PBS containing 0.02% sodium azide.

Stable at 4˚ C for three months, store at -20˚ C for up to one year.

The members of the SAP90/PSD-95-associated protein (SAPAP) family (also known as the DLGAP family) specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. The SAPAP proteins are thought to be adaptor proteins that also interact with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1, -2 and -4 mRNA are targeted to cell bodies, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM). SAPAP3 protein is targeted to dendrites. Recent experiments have suggested that SAPAP3 may be involved in obsessive-compulsive disorder (OCD), as mice lacking SAPAP3 exhibited OCD-like symptoms which could be relieved by lentiviral-mediated selective expression of SAPAP3 in the striatum of SAPAP3-mutant mice. Multiple isoforms of the SAPAP proteins are known to exist.

For images please see PDF data sheet

Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.

Polyclonal

SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. J. Biol. Chem. 1997; 272:11943-51.

Bongiorno-Borbone L, Kadare G, Benfenati F, et al. FAK and PYK2 interact with SAP/PSD-95-associated protein-3. Biochem. Biophys. Res. Commun. 2005; 337:641-6.

SAPAP1 Antibody: Rat Brain Tissue Lysate, Catalog No. 1463

SAPAP2 Antibody: L1210 Cell Lysate, Catalog No. 1284

SAPAP3 Antibody: Rat Brain Tissue Lysate, Catalog No. 1463

SAPAP4 Antibody: L1210 Cell Lysate, Catalog No. 1220

Antibody 1 mg/mL