ArtNr |
PRS-PSI-1826-1Set |
Hersteller |
ProSci
|
Menge |
1 Set |
Kategorie |
|
Typ |
Detection Sets |
Format |
Liquid |
Applikationen |
WB, IF, IHC |
Specific against |
Human |
ECLASS 10.1 |
32161090 |
ECLASS 11.0 |
32161090 |
UNSPSC |
41116126 |
Lieferbar |
|
Applications |
These polyclonal antibodies can be used for detection of SAPAP1 - 4 by immunoblot at 0.5 - 2 μ g/mL, and for detection of SAPAP1 - 3 by immunohistochemistry at 2.5 - 5 μ g/mL, and Immunoflourescence. |
Applications |
These polyclonal antibodies can be used for detection of SAPAP1 - 4 by immunoblot at 0.5 - 2 μ g/mL, and for detection of SAPAP1 - 3 by immunohistochemistry at 2.5 - 5 μ g/mL, and Immunoflourescence. |
Buffer |
PBS containing 0.02% sodium azide. |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Storage Conditions |
Stable at 4˚ C for three months, store at -20˚ C for up to one year. |
Disclaimer |
This product is for research use only. |
Background |
The members of the SAP90/PSD-95-associated protein (SAPAP) family (also known as the DLGAP family) specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. The SAPAP proteins are thought to be adaptor proteins that also interact with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1, -2 and -4 mRNA are targeted to cell bodies, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM). SAPAP3 protein is targeted to dendrites. Recent experiments have suggested that SAPAP3 may be involved in obsessive-compulsive disorder (OCD), as mice lacking SAPAP3 exhibited OCD-like symptoms which could be relieved by lentiviral-mediated selective expression of SAPAP3 in the striatum of SAPAP3-mutant mice. Multiple isoforms of the SAPAP proteins are known to exist.
For images please see PDF data sheet |
Set Contents |
SAPAP1 Antibody, Catalog No. 4623 (50 μ g)
SAPAP2 Antibody, Catalog No. 4625 (50 μ g)
SAPAP3 Antibody, Catalog No. 4411 (50 μ g)
SAPAP4 Antibody, Catalog No. 4633 (50 μ g) |
Immunogen |
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins. |
Purification |
Antibodies are supplied as affinity chromatography purified IgG. |
Clonality |
Polyclonal |
Related Products |
SAPAP1 Peptide, Catalog No. 4623P
SAPAP2 Peptide, Catalog No. 4625P
SAPAP3 Peptide, Catalog No. 4411P
SAPAP4 Peptide, Catalog No. 4633P |
Reference 1 |
SAPAPs. A family of PSD-95/SAP90-associated proteins localized at postsynaptic density. J. Biol. Chem. 1997; 272:11943-51. |
Reference 2 |
Kindler S, Rehbein M, Classen B, et al. Distinct spatiotemporal expression of SAPAP transcripts in the developing rat brain: a novel dendritically localized mRNA. Brain Res. Mol. Brain Res. 2004; 126:14-21. |
Reference 3 |
Bongiorno-Borbone L, Kadare G, Benfenati F, et al. FAK and PYK2 interact with SAP/PSD-95-associated protein-3. Biochem. Biophys. Res. Commun. 2005; 337:641-6. |
Reference 4 |
Welch JM, Wang D, and Feng G. Differential mRNA expression and protein localization of the SAP90/PSD-95-associated proteins (SAPAPs) in the nervous system of the mouse. J. Comp. Neurol. 2004; 472:24-39. |
Positive Control 1 |
SAPAP1 Antibody: Rat Brain Tissue Lysate, Catalog No. 1463
SAPAP2 Antibody: L1210 Cell Lysate, Catalog No. 1284
SAPAP3 Antibody: Rat Brain Tissue Lysate, Catalog No. 1463
SAPAP4 Antibody: L1210 Cell Lysate, Catalog No. 1220 |
Products |
4623, 4625, 4411, 4633 |
Concentration |
Antibody 1 mg/mL |
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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.