ArtNr |
S2417-50 |
Hersteller |
Selleckchem
|
CAS-Nr. |
5289-74-7 |
Menge |
50 mg |
Kategorie |
|
Typ |
Inhibitors |
Specific against |
other |
ECLASS 10.1 |
32160490 |
ECLASS 11.0 |
32160490 |
UNSPSC |
12000000 |
Alias |
Ecdysterone, 20E, B-ecdysone, Commisterone, Crustecdysone, Others |
Similar products |
20-Hydroxyecdysone |
Lieferbar |
|
Administration |
Orally administrated |
Animal Models |
Male C57BL/6J mice fed a high-fat diet |
Chemical Name |
(2S, 3R, 5R, 9R, 10R, 13R, 14S, 17S)-2, 3, 14-trihydroxy-10, 13-dimethyl-17-((2R, 3R)-2, 3, 6-trihydroxy-6-methylheptan-2-yl)-1, 2, 3, 4, 5, 10, 11, 12, 13, 15, 16, 17-dodecahydro-9H-cyclopenta[a]phenanthren-6(14H)-one |
Description |
20-Hydroxyecdysone (Ecdysterone, 20E) is a naturally occurring ecdysteroid hormone which controls the ecdysis (moulting) and metamorphosis of arthropods. |
Dosages |
10 mg/kg/day |
Formulation |
Formulated in vehicle solution (10% DMSO in corn oil) |
In vitro |
In H4IIE cell culture, 20-Hydroxyecdysone treatment decreases expression of phosphoenolpyruvate carboxykinase (PEPCK) and glucose-6-phosphatase (G6Pase), reduces glucose production, and induces Akt2 phosphorylation sensitive to the phosphoinositide-3 kinase pathway-specific inhibitor LY-294002. [1] 20-Hydroxyecdysone treatment before or after CoCl2 exposure significantly ameliorates CoCl2-induced cellular injuries. 20-Hydroxyecdysone dramatically reduces the CoCl2-induced production of reactive oxygen species (ROS), decreases the depolarization of the mitochondrial membrane, inhibits the release of cytochrome c into the cytosol, increases the Bax/Bcl-2 ratio, and eliminates the CoCl2-induced activation of caspase-3. [2] 20-Hydroxyecdysone inhibits NF-kappaB activation in HeLa cells stably transfected with an IL-6-bound reporter gene with IC50 of 31.8 uM. [3] In vitro treatment of larval fat body tissues with 20-Hydroxyecdysone upregulates the apoptotic genes including Apaf-1, Nedd2 like1, Nedd2 like2, ICE1, ICE3, ICE5, Arp, and IAP. [4] 20-Hydroxyecdysone reverses TGF-beta1-induced increase in intracellular and extracellular fibronectin, and reverses TGF-beta1-induced down-regulation of Smad7. Additionally, 20-Hydroxyecdysone significantly attenuates TGF-beta1-induced upregulation of Smad2/3 and pSmad2/3, and downregulation of E-Cadherin. Moreover, 20-Hydroxyecdysone dramatically suppresses TGF-beta1-induced increases in the expression of Snail. [5] |
In vivo |
Oral administration of 20-Hydroxyecdysone (10 mg/kg/day for 13 weeks) ameliorates obesity and insulin resistance in C57BL/6J mice fed a high-fat diet and produces a significant decrease of body weight gain and body fat mass compared with untreated animals. In addition, plasma insulin levels and glucose tolerance are significantly lowered by 20-Hydroxyecdysone treatment. These changes are accompanied by the reduced hepatic expression of PEPCK and G6Pase and increased adiponectin production by visceral fat tissue. [1] In the fat body of the silkworm, Bombyx mori, injection of 20-Hydroxyecdysone into day 2 of fifth instar larvae significantly induces apoptosis and upregulates apoptotic genes after 6 hours of treatment. [4] 20-Hydroxyecdysone (5 mg/kg/day) treatment does not have potent anabolic properties, however, a muscle-specific increase is observed and genes are identified to provide an explanation for the muscle accretion. [6] |
Molecular Weight (MW) |
480, 63 |
Picture ChemicalStructure Description |
20-Hydroxyecdysone Chemical Structure |
Solubility (25C) |
DMSO 96 mg/mL, Water <1 mg/mL, Ethanol 96 mg/mL |
Storage |
2 years -20CPowder, 2 weeks4Cin DMSO, 2 months-80Cin DMSO |
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