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Anti-Mouse CD32/CD16 (2.4G2) In Vivo Antibody - Low Endotoxin Europäischer Partner

ArtNr ICH1056-1MG
Hersteller IchorBio
Menge 1 mg
Quantity options 100 mg 1 mg 25 mg 50 mg 5 mg
Kategorie
Typ Antibody
Applikationen WB, FC, IP, IHC, Blocking
Clon 2.4G2
Specific against Mouse (Murine, Mus musculus)
Host Rat
Isotype IgG2b
Purity >95% by SDS-PAGE and HPLC
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Low affinity immunoglobulin gamma Fc region receptor II, Fcgr2, Fc-gamma-RIIB, FcRII, IgG Fc receptor II beta, Ly-17, Low affinity immunoglobulin gamma Fc region receptor III, Fcgr3, FcRIII, Fc-gamma RIII
Versandbedingung Gekühlt
Lieferbar
Specificity Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) recognizes the FcgammaII and FcgammaIII receptors
Manufacturer - Type
Low Endotoxin, anti-mouse
Manufacturer - Applications
Western Blot, Blocking, Flow cytometry, IHC (Frozen), Immunoprecipitation, CODEX
Manufacturer - Category
Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype
Rat IgG2b
Manufacturer - Targets
CD32/CD16
Shipping Temperature
Blue ice
Storage Conditions
anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation
Aggregation level ≤ 5%
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
The Fc gamma II/III receptors are present on mouse natural killer cells, monocytes, macrophages, granulocytes, B-cells and most fetal thymocytes.
Application Notes
Each investigator should determine their own optimal working dilution for specific applications.
Background
CD16 is a low-affinity IgG Fc receptor III and CD32 is FcR II. CD16/CD32 are expressed on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. The Fc receptors bind antibody-antigen immune complexes and mediate adaptive immune responses. Clone 2.4G2 antibody is used in flow cytometry staining experiments to prevent non-specific binding of IgG to the FcγIII and FcγII, and possibly FcγI, receptors prior to staining with antigen-specific primary antibodies. The Fab fragments of the 2.4G2 antibody have also been used to block Fc receptors in vivo.
Concentration
0.5 mg/ml
Endotoxin
≤ 1.0 EU/mg as determined by the LAL method
Manufacturer - Specificity
Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin (2.4G2) recognizes the FcγII and FcγIII receptors
Purification method
This monoclonal antibody was purified using Protein G
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Antibodies against the same target (Cat No.)
Isotype Control
Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)
Buffer
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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