Anti-Rac1 specific mouse MAb

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Rac1 is one of most extensively studied member of Rho GTPase family. This family is involved in a wide range of cellular responses, including cytoskeletal reorganization, regulation of transcription, cell migration, cellular transformation and metastasis. Rac1 is expressed in a large number of different cell types.

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The anti-Rac1 antibody (Cat. # ARC03) is a mouse monoclonal antibody that only recognizes Rac1, not Rac2, Rac3, Cdc42 or other small GTPases. Other commercially available Rac1 antibodies recognize Rac2 and 3, some even recognize Cdc42 (Table 1 and Figure 1). Platelet extract (Cat. # EXT01) is included as a positive control. ARC03 is supplied as a lyophilized white powder.

Figure 1 legend - Western blot of purified Rac1, 2, 3, Cdc42 and platelet extracts probed with anti-Rac1 monoclonal antibody (Cat. # ARC03) and other commercially availabe Rac1 antibodies. The detection of 25 ng His-Rac1 ( lane 1), His-Rac2 (lane 2), GST-Rac3 (lane 3), GST-Cdc42 (lane 4) and 50 ug of platelet extract (lane 5). ARC03 does not cross-react with Rac2, 3 or Cdc42, while all other commercially available Rac1 antibody crossreact with GTPases other than Rac1. The blot was probed with a 1 ug/ml (1:500) dilution of ARC03. Other blots were probed according to manufacturers' protocols.