Precision Red Advanced Protein Assay (1X conc)

On Arrival: 4°C

Question 1: Can I change the dilution volume of the experimental lysate being assayed?

Answer 1: Yes, different volumes of lysate can be used to quantify protein levels. The equation to determine protein concentration in µ, g/ml is: OD reading x dilution factor x 100 µ, g/ml/cm if a small volume (1.0 ml) cuvette is used. If a 96 well plate is used, the same equation is used with this single modification: 125 µ, g/ml/cm is the value used rather than 100 µ, g/ml/cm.

Question 2: Can this assay be performed using 96 well plates?

Answer 2: Yes, the protein assay can be performed in either small volume cuvettes (1.0 ml) or a 96 well plate.If a 96 well plate is used, the equation to determine protein concentration in µ, g/ml is: OD reading x dilution factor x 125 µ, g/ml/cm.

Product uses include measuring protein in
Cell extracts
Serum samples
Medium to high protein concentration solutions
Protein requiring GLP and higher standards
Purified proteins and antibodies

The Precision Red Advanced Protein Assay makes it very easy and quick to measure prottein concentrations. A general method looks as follows:
Mix protein solution with ADV02 reagent (e.g. 10 ul sample with 1.0 ml ADV02)
Incubate 1 min
Read absorbance at 600 nm

Since the Precision Red Advanced Protein Assay is highly compatible with non-ionic detergents (see the ADV Chemical tolerance page), this reagent is ideal for measuring protein concentrations in cell and tissue extracts. Furthermore, since protein concentration can be measured after only 1 min of incubation with the reagent, it allows the user to proceed with the experiment quicker than any other protein assay, something that can be of big importanceWhen working with labile cell extracts.
Cytoskeleton, Inc. recommends using ADV02 for all our kits and assays that involve cell or tissue lysis. In the G-LISA small G-protein activation kits, we have even included this reagent to ensure that the user gets the most accurate and quickest possible measurement of the protein concentration in the cell lysates generated.