APOBEC3G (apolipoprotein B mRNA editing enzyme. catalytic polypeptide-like 3G)

Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human APOBEC3G. Western Blot: Suggested dilution at 2. 5 microg/ml in 5% skim milk / PBS buffer. and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50. 000 - 100. 000 as second antibody.

RNA Binding Proteins

DNA deaminase (cytidine deaminase) that mediates a form of innate resistance to retroviral infections (at least to HIV-1 infection) by triggering G-to-A hypermutation in the newly synthesized viral DNA. The replacements C-to-U in the minus strand DNA of HIV-1 during reverse transcription leads to G-to-A transitions in the plus strand. The inhibition of viral replication is either due to the degradation of the minus strand before its integration or to the lethality of the hypermutations. Modification of both DNA strands is not excluded. This antiviral activity is neutralized by the virion infectivity factor (VIF) that prevents the incorporation of APOBEC3G into progeny HIV-1 virions by both inhibiting its translation and/or by inducing its ubiquitination and subsequent degradation by the 26S proteasome. APOBEC3G binds a variety of RNAs but does not display detectable APOB NF1 and NAT1 mRNA editing. Cofactor: Zinc.

Cytoplasm. Nucleus. Note=Mainly cytoplasmic. Small amount are found in the nucleus. During HIV-1 infection virion-encapsidated in absence of HIV-1 VIF.

Ubiquitinated in the presence of HIV-1 VIF. Association with VIF targets the protein for proteolysis by the ubiquitin-dependent proteasome pathway.

HIV-1 does not replicate productively in nonhuman primates with the exception of the chimpanzee. The primates APOBEC3G (rhesus macaque chimpanzee and African green monkey) are active against HIV-1 without VIF. Only the chimpanzee APOBEC3G is inhibited by HIV-1 VIF.

It is one of seven related genes or pseudogenes found in a cluster thought to result from gene duplication on chromosome 22.