Vergleich

Hepatitis B surface antigen

ArtNr 20-902-170375
Hersteller GENWAY
Menge 0,1 mg Purified
Typ Antibody
Specific against other
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias GWB-629C50
Similar products 20-902-170375
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Genway ID:
GWB-629C50
Agent:
Hepatitis B virus
Specificity:
sAg. Probe
Ig Class:
IgG2a
Purified Preparations:
MONOTOPE purified preparations consist of > 90% pure mouse monoclonal antibody which has been purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations. The final preparation is formulated to a protein concentration of 100 ?g/ml in 0. 01 M phosphate buffered saline pH 7. 2 and contains 0. 1% sodium azide. Each vial contains 1. 0 ml. This product contains no stabilizing proteins and should be stored at -20° C until ready for use. Avoid repeated freeze-thawing by storing multiple aliquots at -20° C. Custom conjugation of this antibody with HRP alkaline phosphatase biotin and fluorescein is available on a minimum order basis
Specificity Note:
Specific to surface antigen; subtypes ad & ay. High affinity probe antibody (ELISA)
Conjugation:
Purified
Function:
The large envelope protein exists in two topological conformations one which is termed \' external\' or Le-HBsAg and the other \' internal\' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. It also assume fusion between virion membrane and cellular membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein (By similarity).
Function:
The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid (By similarity).
Subunit:
Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion (By similarity).
Subcellular Location:
Virion membrane (By similarity).
Domain:
The large envelope protein is synthesized with the pre-S region at the cytosolic side of the endoplasmic reticulum and hence will be within the virion after budding. Therefore the pre-S region is not N-glycosylated. Later a post-translational translocation of N-terminal pre-S and TM1 domains occur in about 50% of proteins at the virion surface. These molecules change their topology by an unknown mechanism resulting in exposure of pre-S region at virion surface. For isoform M in contrast the pre-S2 region is translocated cotranslationally to the endoplasmic reticulum lumen and is N-glycosylated.
Ptm:
Isoform M is N-terminaly acetylated at a ratio of 90% N- and O-glycosylated at the pre-S2 region.
Ptm:
Myristoylated (By similarity). Biotechnology: Systematic vaccination of individuals at risk of exposure to the virus has been the main method of controlling the morbidity and mortality associated with hepatitis B. The first hepatitis B vaccine was manufactured by the purification and inactivation of HBsAg obtained from the plasma of chronic hepatitis B virus carriers. The vaccine is now produced by recombinant DNA techniques and expression of the S isoform in yeast cells. The pre-S region do not seem to induce strong enough antigenic response.
Similarity:
Belongs to the orthohepadnavirus major surface antigen family.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0,1 mg Purified
Lieferbar: In stock
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