Mouse Anti-Mouse Ly49F

Mouse (BALB/c) IgG1 kappa

CHO cells transfected with the B6 allele of Ly49F 1

B6 allele of the Ly49F receptorThe monoclonal antibody HBF-719 reacts with the B6 allele of Ly49F a member of the lectin-like homodimeric Ly49 family of cell surface receptors. 1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated. 2 These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells. The HBF-719 antibody stains ~ 11% of NK1. 1+CD3- splenocytes and 3. 5% (2. 5 month old) to 10. 0% (11 month old) of CD8+ spleen cells from C57BL/6 mice. 1

Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin eyes or clothing. Wear eye or face protection when handling. If skin or eye contact occurs wash with copious amounts of water. If ingested or inhaled contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Homodimer; disulfide-linked.

Contains 1 C-type lectin domain. 1. Coles M. C. C. W. McMahon H. Takizawa and D. H. Raulet. 2000. Eur. J. Immunol. 30:236. 2. Ljunggren H. G. and K. Karre. 1990. Immunol. Today 11:237. 3. Liu S. and D. H. Raulet. Personal communication.