Vergleich

TCR ALPHA/BETA

ArtNr 20-783-74376
Hersteller GENWAY
Menge 0.5 mg
Kategorie
Typ Antibody
Applikationen FC, IP, IHC
Clon R73
Specific against other
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias GWB-51A69B
Similar products 20-783-74376
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Genway ID:
GWB-51A69B
Specificity:
TCR ALPHA/BETA
Specificity:
TCR ALPHA/BETA
Clone:
R73
Immunogen:
Rat T Blasts and Erythrocytes
Fusion Partner:
Spleen cells from immunised BALB/c mice were fused with cells of the X63. Ag8. 653 mouse myeloma cell line.
Specificity Note:
This product recognises a constant determinant on the beta chain of the rat alpha/beta T cell receptor expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. Clone R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus mature medullary cells express the R73 determinant at the same levels as peripheral T cells whereas 94% of CD4 - CD8 - thymocytes are R73 negative. This clone is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (2).
Preparation:
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution:
Phosphate buffered saline pH7. 4
Preservative Stabilisers:
None presentSuggested
Flow Cytometry:
Use 10ul of the suggested working dilution to label 100ul of whole blood or 106 cells in 100ul. Suggested
Dilution:
Flow Cytometry - 1/50 - 1/100Immunohistology - Frozen - 1/10 - 1/100 Miscellaneous: This sequence was derived from a human cytotoxic T-lymphocyte that is T3+ T4+ T8-. 1. Hunig. T. et al. (1989) A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes 2. Tsuchida. M. et al. (1994) Identification of CD4- CD8- alpha/beta T cells in the subarachnoid space of rats with experimental autoimmune encephalomyelitis. A possible route by which effector cells invade the lesions. 3. Tomida. S. et al. (1994) Intercellular adhesion molecule-1 and leukocyte function-associated antigen-1 are involved in protection mediated by CD3+TCR alpha beta- T cells at the early stage after infection with Listeria monocytogenes in rats 4. Matsumoto. Y. et al . (1994) Successful prevention and treatment of autoimmune encephalomyelitis by short term administration of anti-T-cell receptor alpha beta antibody. 5. Colic. M. et al. (1996) Mechanisms involved in the binding of thymocytes to rat thymic dendritic cells. 6. Ariki. S. G. and Roux. M. E. (0) T lymphocyte subpopulations in the intestinal villi of immune deficient rats. In Recent Advances in Mucosal Immunology. Edited by McGhee. J. . Mestecky. J. . Tlaskalova. H. and Sterzl. J. 7. Yoshino. N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts. cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 0.5 mg
Lieferbar: In stock
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