CD284

CD284NCBI

CD284

Ba/F3 cell line expressing TLR4 (CD284).

This product recognises the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4 also known as CD284 has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells but is upregulated following stimulation with LPS. This antibody has been demonstrated to block activation of monocytes with LPS. We recommend the use of for this purpose.

Phosphate buffered saline

Use 10ul of the suggested working dilution to label 106 cells or cells or 100ul whole blood. 1. Shimazu. R. et al. (1999) MD-2. a molecule that confers lipopolysaccharide responsiveness on Toll like receptor 4. 2. Jiang. Q. et al. (2000) Cutting edge: lipopolysaccharide induces physical proximity between CD14 and Toll like receptor 4 (TLR4) prior to nuclear translocation of NF-Kappa B. 3. Jiang. Q. et al. (2000) Lipopolysaccharide induces physical proximity between CD14 and Toll like receptor 4 (TLR4) prior to nuclear translocation of NF-Kappa B. 4. Yang. S. et al. (2001) Synergistic effect of muramyldipeptide with lipopolysaccharide or lipoteichoic acid to induce inflammatory cytokines in human monocytic cells in culture. 5. Triantafilou. M. et al. (2002) Mediators of innate immune recognition of bacteria concentrate in lipid rafts and facilitate lipopolysaccharide-induced cell activation. 6. Kawahara. T. et al. (2001) Type I Helicobacter pylori lipopolysaccharide stimulates toll-like receptor 4 and activates mitogen oxidase 1 in gastric pit cells.