CD45RO

IgG2a

IL-2 dependent T-cell line CA1

Monoclonal Antibodies to Human CDs

0. 01M PBS pH 7. 4 containing 1% BSAApplications Notes : When activated with phytohemagglutinin (PHA) naive T-cells lose the CD45RA antigen and begin to express the CD45RO antigen. Therefore Anti-CD45RO may be a marker for memory T-cells. Anti-CD45RO may be used in formalin-fixed and paraffin-embedded tissues as well as frozen sections and cell suspensions. We recommend using 1ug to stain 1. 0 x 106 cells in flow cytometric applications. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation recruits an dephosphorylates SKAP1 and FYN.

Binds GANAB and PRKCSH (By similarity). Interacts with SKAP1.

The first PTPase domain interacts with SKAP1.

Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)B(+)NK(+)SCID) [MIM:608971]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity leukopenia and low or absent antibody levels. Patients with SCID present in infancy with recurrent persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.

Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.

Contains 2 tyrosine-protein phosphatase domains.