1A antigen [MRC OX-3] (FITC)

MRC OX-3

Rat thymocyte membrane glycoprotein

Recognizes a polymorphic determinant on rat Ia present on Lewis Wistar and AO rat strains (although it is apparently expressed in lower amounts in AO rats) but not BN DA or PVG/c strains. This antibody also cross-reacts with mouse strains (MHC haplotypes b and s) and analysis of recombinant mouse strains shows that the determinant maps to the I-A region and correlates with mouse Ia specificity 9. Recognizes Ia antigens on approximately 18% of thymocytes but does not significantly label the majority of peripheral T lymphocytes. Is particularly useful for distinguishing Ia positive cells from different rat strains and has been used in the recognition of cells of donor origin in bone marrow reconstituted radiation chimeras.

FITC

From mouse ascitic fluid Storage

For FACS: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. Myeloma: Spleen cells from immunised BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.

Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein (By similarity).

Sulfated. Polyubiquitinated in a post ER compartment through interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).

Belongs to the MHC class I family.