CD7 [LT7]

LT7

Normal human peripheral blood lymphocytes

Recognizes CD7 a single chain cell surface glycoprotein of 40kD which is expressed by T cells NK cells and thymocytes. CD7 is also expressed by tumour cells in some cases of acute myeloid leukaemia.

1 mg/ml Storage

For FACS: Use at a dilution of 1:50 - 1:100. Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. For IHC-Fr and IP: Use at an assay dependent dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Myeloma: Spleen cells from immunised BALB/c mice were fused with cells of the X63. 653 mouse myeloma cell line. CD7 is a 40 kDa transmembrane single-chain glycoprotein which is a member of the immunoglobulin superfamily. CD7 is expressed by the majority of thymocytes and mature T cells NK cells and pre-B cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development. The function of CD7 is not yet known although cross-linking of CD7 with antibodies induces a T cell transmembrane calcium flux and CD7 expression is induced by ionomycin. The CD7 molecule has been reported to be the receptor for the IgM-Fc portion (FcR mu) on the surface of T cells.

Interacts with SECTM1.

Contains 1 Ig-like (immunoglobulin-like) domain.