ArtNr |
20-272-193223 |
Hersteller |
GENWAY
|
Menge |
0.1 mg |
Kategorie |
|
Typ |
Antibody |
Applikationen |
WB, IF, IP |
Clon |
2B5 |
Specific against |
other |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
GWB-6C36D0 |
Similar products |
20-272-193223 |
Lieferbar |
|
Genway ID: |
GWB-6C36D0 |
Clone: |
2B5 |
Isotype: |
IgG |
Immunogen: |
Recombinant fragment corresponding to amino acids 710-1100 of Human ATR. |
Antigen Species: |
Human |
Positive Control: |
Raji whole cell lysate |
Target: |
ATR |
Concentration: |
1 mg/ml |
Purification Note: |
From Ascites Storage |
Buffer: |
Phosphate-buffered saline pH7. 2 |
Application Note: |
For WB: Use at a dilution of 1:500-1 000. For IP and IF information please refer to publications. Not yet tested in other applications. Optimal dilution should be determind by the researcher. Target protein |
Molecular Weight: |
317 Myeloma: NS1 Ataxia Telangiectasia Mutated (ATM) and Rad3-related (ATR) protein is a member of the phosphatidyl-inositol 3-kinase (PI 3-kinase) like family of protein kinases. ATR has a predicted molecular mass of approximately 301 kDa and it has been shown to be involved in cellular responses to DNA damage. Specifically it plays a role in checkpoint activation in response to either the presence of stalled replication forks or by the presence of lesions caused by UV light. Inactivation of ATR is an embryonic lethal event in mice. |
Function: |
Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR) ultraviolet light (UV) or DNA replication stalling thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1 CHEK1 MCM2 RAD17 RPA2 SMC1 and TP53/p53 which collectively inhibit DNA replication and mitosis and promote DNA repair recombination and apoptosis. Phosphorylates \' Ser-139\' of histone variant H2AX/H2AFX at sites of DNA damage thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. |
Catalytic Activity: |
ATP + a protein = ADP + a phosphoprotein. Cofactor: Manganese. |
Enzyme Regulation: |
Activated by DNA and inhibited by BCR-ABL oncogene. Slightly activated by ATRIP. Inhibited by caffeine wortmannin and LY294002. |
Subunit: |
Forms an heterodimer with ATRIP. Binds to DNA and to UV-damaged DNA with higher affinity. Interacts with RAD17 MSH2 and HDAC2. Present in a complex containing ATRIP and RPA-coated single-stranded DNA. Present in a complex containing CHD4 and HDAC2. Interacts with BCR-ABL after genotoxic stress. Interacts with EEF1E1. This interaction is enhanced by UV irradiation. Interacts with CLSPN. |
Subcellular Location: |
Nucleus. Note=Depending on the cell type it can also be found in PML nuclear bodies. Recruited to chromatin during S-phase. Redistributes to discrete nuclear foci upon DNA damage hypoxia or replication fork stalling. |
Tissue Specificity: |
Ubiquitous with highest expression in testis. Isoform 2 is found in pancreas placenta and liver but not in heart testis and ovary. |
Ptm: |
Phosphorylated; autophosphorylates in vitro. |
Disease: |
Defects in ATR are a cause of Seckel syndrome 1 (SCKL1) [MIM:210600]. SCKL1 is a rare autosomal recessive disorder characterized by growth retardation microcephaly with mental retardation and a characteristic \' bird-headed\' facial appearance. |
Similarity: |
Belongs to the PI3/PI4-kinase family. ATM subfamily. |
Similarity: |
Contains 1 FAT domain. |
Similarity: |
Contains 1 FATC domain. |
Similarity: |
Contains 2 HEAT repeats. |
Similarity: |
Contains 1 PI3K/PI4K domain. |
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