CDC2 [3E1.1]

GWB-491AC5

IgG1

Xenopus

CDC2

1 mg/ml Storage

WB: Use at an assay dependent dilution. Predicted

Nuclear Myeloma: Sp2 The cell division control protein (cdc2) kinase is a catalytic subunit of a protein kinase complex called the M phase promoting factor that induces entry into mitosis and is universal among eukaryotes. In the fission yeast Schizosaccharomyces pombe the gene Cdc2 is responsible for controlling the transition from G1 phase to the S phase and from the G2 phase to the M phase of the cell cycle. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex that is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase.

ATP + a protein = ADP + a phosphoprotein.

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme while phosphorylation at Thr-161 activates it.

Nucleus (By similarity).

Contains 1 protein kinase domain.