PPAR alpha [3B6/PPAR]

3B6/PPAR

Recombinant full length PPAR alpha protein.

Nuclear

Phosphate buffered saline containing 0. 05% sodium azide

Nuclear Peroxisome proliferators are non genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid nafenopin and WY-14 643 as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic arachidonic and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle heart liver and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha like several other nuclear hormone receptors heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.

Heterodimer with the retinoid X receptor. Interacts with NCOA3 and NCOA6 coactivators leading to a strong increase of transcription of target genes. Also interacts with PPARBP coactivator in vitro. Interacts with AKAP13 (By similarity).

Skeletal muscle liver heart and kidney.

Contains 1 nuclear receptor DNA-binding domain.