Pim1

GWB-4CD6AA

A synthetic peptide corresponding to residues in human Pim1 was used as a immunogen.

WB: 1:1 000 - 10 000IHC: 1:100 - 250ICC: 1:100 - 250. Protein kinase Pim-1 is a serine/threonine kinase that has been implicated in the development of hematopoietic and prostatic malignancies. Two isoforms the 44 and 33 kDa Pim-1 are expressed in all human prostate cancer cell lines examined. The subcellular localization of human 44 kDa Pim-1 is primarily on the plasma membrane while the 33 kDa isoform is present in both the cytosol and nucleus in PCA cells (1). The nuclear location of Pim-1 is essential for its regulation of the levels of HDM2 protein and possibly for additional biological activities of this protein kinase. Studies imply a physiological role of the Pim-1 protooncogene during hematopoietic development and a deregulation in various leukemias. Pim-1 is capable of enhancing the rate of occurrence of c-Myc-induced lymphomas and functions to block factor-withdrawal and genotoxic stress-induced apoptosis. During human fetal hematopoiesis Pim-1 is highly expressed in the liver and spleen. In contrast at the adult stage it is only slightly expressed in circulating granulocytes (2-3). 1. Xie Y et al. Oncogene 25(1):70-8 20062. Ionov Y et al. FEBS Lett 467(1):17-21 20003. Amson R et al. Proc Natl Acad Sci 86(22):2257-61 19894. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487