EGF Receptor (Pan)

GWB-D51808

A synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGF receptor was used as immunogen. The antibody detects both EGFR phosphorylated on Tyrosine 1068 of the mature human isoform 1 (corresponding to Y1092 from the precursor form P00533-1/p170) and also unphosphorylated EGFR.

WB: 1:100 000IHC: 1:100 250ICC: 1:250 500IP: 1:50. Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that activated by epidermal growth factor (EGF) affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR Tyr 1068 Tyr 1148 and Tyr 1173 are the major sites of autophosphorylation which occurs as a result of EGF binding (3). Once activated EGFR phosphotyrosines Y-1068 and Y-1173 mediate the binding of Grb2 to the EGFR. Addtionally phosphotyrosine Y-1173 is a major binding site for SHC on the EGFR (4). 1. Hunter T et al. Nature 311: 4142. Reynolds F. H. Jr. et al. Nature 292: 2593. Helin K. et al. Oncogene 6: 8254. Batzer A. G. et al. Mol. Cell. Biol. 14: 51925. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487