MKK5

GWB-86A459

A synthetic peptide corresponding to residues within human MKK5 was used as an immunogen.

WB: 1:1 000 - 10 000ICC: 1:100-250. Mitogen-acitvated protein kinase kinase 5 (MKK5) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues an activation step carried out by MAPK kinase (1-2). The MAPK cascade plays an important role in signaling defense responses. Of the MKK family members MKK5 exhibits the closest homology to MEK1 and MEK2 with 46% amino acid identity in the catalytic domain (3) MKK has been show to activate extracellular-signal-regulated protein kinase 5 (ERK5). Also activated ERK5 phosphorylates mitogen- MKK5 extensively at Ser129 Ser137 Ser142 and Ser149 which are located within the region in MKK5 that is thought to interact with ERK5 (3). There have also been findings to that the MEK5 (MKK5)/ERK5 (BMK) pathway may play a role in prostate cancer (5). 1. Han J et al. J Bio Chem 271:2886-2891 19962. Seger R et al. FASEB J. 9:726-735 19953. Mody N et al. FEBS Lett 502:21-24 20014. Mody N et al. J. Bio Chem 372:567-575 20015. Mehta PB et al. Oncogene 22:1381-1389 20036. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487