MEKK3 (C-term)

GWB-928B85

A synthetic peptide corresponding to residues in the C-term of human MEKK3 was used as immunogen.

WB: 1:500-1 000IHC: 1:100-250ICC: 1:100-250FC: 1:20IP: 1:20. MEKK3 is a Ser/Thr protein kinase belonging to the MAP/ERK kinase kinase family (1). Closely related to MEKK2 MEKK3 is expressed in a multitude of tissues. MEKK3 directly regulates SAPKs and ERKs pathway via SEK and MEK1 respectively but not the p38 pathway (2) and is also involved in the NF-kB pathway (3). MEKK3 as well as MEKK2 bind phosphorylate and activate MEK5 through their PB1 domain (4). hKSR-2 (human kinase suppressor of ras-2) has been shown to regulate the activity of MEKK3 (5). 1. Fakler M Loeder S Vogler M et al Small molecule XIAP inhibitors cooperate with TRAIL to induce apoptosis in childhood acute leukemia cells and overcome Bcl-2-mediated resistance Blood 113 (2009) 1710 - 1722[Application: ]2. Sumithra Urs S Roudabush A Oâ ??Neill CF et al Soluble Forms of the Notch Ligands Delta1 and Jagged1 Promote in Vivo Tumorigenicity in NIH3T3 Fibroblasts with Distinct Phenotypes The American Journal of Pathology 173 (2008) 865-878[Application: WB]3. Son M Kim J Han H et al Expression profiles of protein tyrosine kinase genes in human embryonic stem cells Reproduction 136 (2008) 423-432[Application: WB]4. Matitau AE Scheid MP Phosphorylation of MEKK3 at Threonine 294 Promotes 14-3-3 Association to Inhibit Nuclear Factor B Activation J. Biol. Chem. 283 (2008) 13261 - 13268[Application: IP]5. Halaban R Zhang W Bacchiocchi A et al PLX4032 a Selective BRAF(V600E) kinase inhibitor activates the ERK pathway and enhances cell migration and proliferation of BRAFWT melanoma cells Pigment Cell Melanoma Res 23 (2010) 190-200[Application: IP]1. Blank J. L. P. Gerwins E. M. Elliott S. Sather and G. L. Johnson. 1996. ; J. Biol. Chem. 271:5361-5368. 2. Zhang et al. : J. Biol. Chem. 272: 2668-2674 1997. 3. Zhao Q. and Lee F. S. (1999) J. Biol. Chem. 274 83554. Wilson M. I. Gill D. J. Perisic O. Quinn M. T. and Williams R. L. (2003) Mol. Cell 12 395. P. L. Channavajhala L. Wu J. W. Cuozzo J. P. Hall W. Liu L. L. Lin Y. Zhang J. Biol. Chem. 278 (2003) 47089-470976. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487