EGFR Phospho (pY1173)

GWB-A46E09

A synthetic phospho-peptide corresponding to residues surrounding Tyr1173 of human EGF receptor was used as immunogen. The antibody detects EGFR phosphorylated on Tyrosine 1173 of the mature human isoform 1 (corresponding to Y1197 from the precursor form

WB: 1:1 000IHC: 1:25-50ICC: 1:25-50IP: 1:10. Epidermal growth factor receptor (EGFR) is a 170 kDa transmembrane glycoprotein receptor tyrosine kinase that activated by epidermal growth factor (EGF) affects cell growth and differentiation (1). Binding of EGF or TGF alpha to EGFR activates tyrosine kinase activity of the receptor (2). The carboxy terminal tyrosine residues on EGFR Tyr 1068 Tyr 1148 and Tyr 1173 are the major sites of autophosphorylation which occurs as a result of EGF binding (3). Once activated EGFR phosphotyrosines Y-1068 and Y-1173 mediate the binding of Grb2 to the EGFR. Addtionally phosphotyrosine Y-1173 is a major binding site for SHC on the EGFR (4). 1. Tavalai N Stamminger T New insights into the role of the subnuclear structure ND10 for viral infection Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1783 (2008) 2207-2221[Application: WB]2. Preuà ? I Kurig B Nurnberg B et al Pasteurella multocida toxin activates GÎ ² Î ³ dimers of heterotrimeric G proteins Cellular Signalling 21 (2009) 551-558[Application: WB]3. Fu KY Tan YH Sung B et al Peripheral Formalin Injection Induces Unique Spinal Cord Microglial Phenotypic Changes Neuroscience Letters 449 (2009) 234-239[Application: IHC]4. Kumar N Afeyan R Kim HD et al Multipathway Model Enables Prediction of Kinase Inhibitor Cross-Talk Effects on Migration of Her2-Overexpressing Mammary Epithelial Cells Mol Pharmacol 73 (2008) 1668-1678[Application: ICC]5. Pickl M Ries CH Comparison of 3D and 2D tumor models reveals enhanced HER2 activation in 3D associated with an increased response to trastuzumab Oncogene 28 (2009) 461-468[Application: WB]1. Hunter T. The epidermal growth factor receptor gene and its product. Nature 311: 4142. Reynolds F. H. Jr. et al. Human transforming growth factors induces tyrosine phosphorylation of EGF receptors. Nature 292: 2593. Helin K. et al. The biological activity of the human epidermal growth factor receptor is positively regulated by its C-terminal tyrosines. Oncogene 6: 8254. Batzer A. G. et al. Hierarchy of binding site for Grb2 and Shc on the epidermal growth factor receptor. Mol. Cell. Biol. 14: 51925. Manufactured under U. S. Patents No. 5 675 063 and 7 429 487