Anti-PhosphoThreonine (pT)

PhosphoThreonine (pT)

This affinity-purified antibody is suitable for use in ELISA western blotting and immunoprecipitation assays. The antibody reacts specifically with phosphothreonine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphoserine residues. The antibody reacts with free phosphothreonine phosphothreonine conjugated to carriers such as thyroglobulin or BSA and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell lysates. Although not tested this antibody is likely functional in RIA flow cytometry and immunohistochemistry. Recommended

This product was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated threonine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG. Reactivity is specific for phosphothreonine and minimal cross reactivity is observed against phosphotyrosine or phosphoserine. Antibody

0. 02 M KPhosphate 0. 15 M NaCl pH 7. 2

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