TACE

GWB-F07C5D

Synthetic peptide corresponding to the cytoplasmic domain of full length TACE (Human).

This antibody does not react with other ADAMs.

Type I membrane protein.

0. 05% Sodium Azide; Constituents: 50% Glycerol PBS

Type I membrane protein. TNF alpha is a pro-inflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. It is synthesized as a 26K type II membrane-bound precursor that is cleaved by aconvertase to generate secreted 17K mature TNF alpha. TNF alpha converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins including TNF receptor TGF alpha the L-selectin adhesion molecule and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.

Narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound 26-kDa form of tumor necrosis factor alpha (TNF-alpha). Similarly cleaves other membrane-anchored cell-surface proteins to \' shed\' the extracellular domains. Cofactor: Binds 1 zinc ion per subunit.

Membrane; Single-pass type I membrane protein.

In arthritis-affected cartilage.

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.

Contains 1 peptidase M12B domain [view classification].