Bcl-X

ArtNr	18-272-195319
Hersteller	GENWAY
Menge	0,05 ml
Typ	Antibody
Applikationen	WB, IHC
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-373A65
Similar products	18-272-195319
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Genway ID:	GWB-373A65
Isotype:	IgG
Immunogen:	Synthetic peptide.
Specificity:	This product recognises the Bcl X apoptosis related protein binding to both the Bcl XL and Bcl XS forms. No cross-reactivity to other Bcl 2 family members (including Bcl-2 Bax and Mcl-l) is seen. In Western blotting applications this antibody may be used to differentiate Bcl-XL (a 30kD protein often appearing as a doublet) from Bcl-XS (a 20kD protein). Bcl-XS lacks a 63 amino-acid region corresponding to residues 126-188 of Bcl-XL.
Positive Control:	Tonsil reactive lymphnode
Target:	Bcl-X
Localization:	Mitochondrial membranes and perinuclear envelope Storage
Preservative:	0. 09% Sodium Azide
Application Note:	For IHC-P: Use at a dilution of 1:200 - 1:1 000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For WB: Use at a dilution of 1:1 000 - 1:2 000. Detects a band of approximately 20-30 kDa (predicted
Molecular Weight:	20. 4 kDa). Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Target protein
Molecular Weight:	30kD Cellular
Localization:	Mitochondrial membranes and perinuclear envelope Bcl-X is a member of the Bcl-2 family of proteins which function as regulators of apoptosis. Bcl-X has two isoforms Bcl-Xl (long) a 241 amino acid protein and Bcl-Xs (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl-2 family.
Function:	Potent inhibitor of cell death. Isoform Bcl-X(L) anti-apoptotic activity is inhibited by association with SIVA isoform 1. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator cytochrome c from the mitochondrial membrane. The Bcl-X(S) isoform promotes apoptosis.
Subunit:	Bcl-X(L) forms homodimers and heterodimers with BAX BAK and BCL2. Heterodimerization with BAX does not seem to be required for anti-apoptotic activity. Also interacts with BAD and BBC3. Isoform Bcl-X(L) binds to Siva isoform 1. Interacts with BCL2L11 (By similarity). Interacts with BECN1 and PGAM5.
Subcellular Location:	Mitochondrion membrane; Single-pass membrane protein (By similarity). Nucleus membrane; Single-pass membrane protein; Cytoplasmic side (By similarity). Note=Mitochondrial membranes and perinuclear envelope (By similarity).
Tissue Specificity:	Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover such as developing lymphocytes. In contrast Bcl-X(L) is found in tissues containing long-lived postmitotic cells such as adult brain.
Domain:	The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
Ptm:	Proteolytically cleaved by caspases during apoptosis. The cleaved protein lacking the BH4 motif has pro-apoptotic activity.
Similarity:	Belongs to the Bcl-2 family.

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