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PPP1R8 (protein phosphatase 1. regulatory (inhibitor) subunit 8)

PPP1R8 (protein phosphatase 1. regulatory (inhibitor) subunit 8)

ArtNr	18-003-44217
Hersteller	GENWAY
Menge	0.1 mg
Kategorie	Oncology Antibodies Western Blot Primary Antibodies By Organ Lung Cancer
Typ	Antibody
Applikationen	WB
Specific against	other
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-179EA8
Similar products	18-003-44217
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Genway ID:	GWB-179EA8
Antigen Specificity:	Polyclonal antibody produced in rabbits immunized with a synthetic peptide corresponding to a region of Human PPP1R8.
Immunogen:	THGTFLGHIRLEPHKPQQIPIDSTVSFGASTRAYTLREKPQTLPSAVKGDLyophilized powderWestern Blot: Antibody
Dilution:	1. 25ug/ml
Handling:	Add 100ul of distilled water to this antibody before use.
ELISA Titre:	1:312500
Note:	Suggested starting concentrations are provided. Optimal dilutions should be determined by end-user. Differences in calculated versus apparent molecular weight may be due to post-translational modifications or protein hydrophobicity. This gene. through alternative splicing. encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform
Function:	Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation.
Function:	Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3\' to purines and pyrimidines in A+U-rich regions. It generates 5\' -phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing. Cofactor: Magnesium. Endoribonuclease function is magnesium-dependent.
Subunit:	Interacts with phosphorylated CDC5L SF3B1 and MELK. Interacts with EED in a nucleic acid-stimulated manner. Part of a complex consisting of PPP1R8 EED HDAC2 and PP-1. Part of the spliceosome.
Subcellular Location:	Nucleus. Nucleus speckle. Note=Primarily but not exclusively nuclear.
Subcellular Location:	Isoform Gamma: Cytoplasm. Note=Found mainly in the cytoplasm.
Tissue Specificity:	Ubiquitously expressed with highest levels in heart and skeletal muscle followed by brain placenta lung liver and pancreas. Less abundant in kidney. The concentration and ratio between isoforms is cell-type dependent. Isoform Alpha (& gt; 90%) and isoform Beta were found in brain heart and kidney. Isoform Gamma is mainly found in B-cells and T-lymphocytes and has been found in 293 embryonic kidney cells.
Domain:	Has a basic N- and C-terminal and an acidic central domain.
Ptm:	May be inactivated by phosphorylation on Ser-199 or Ser-204 (By similarity). Phosphorylated by Lyn in vitro on Tyr-264 and also on Tyr-335 in the presence of RNA.
Miscellaneous:	A synthetic peptide NIPP-1(330-351) is able to inhibit PP-1. Phosphorylation of Tyr-335 reduces PP-1 inhibition whereas phosphorylation of Thr-346 or Ser-348 has no effect.
Similarity:	Contains 1 FHA domain. Summary: This protein through alternative splicing encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA. Lesage. B. . (2004) J. Biol. Chem. 279 (53). 55978-55984.

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Menge: 0.1 mg

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