Rat Anti-Mouse CD25/IL-2R

Rat (Lewis) IgM kappa

IL-2-dependent BALB/c mouse helper T-cell clone HT-2 1

Low affinity chain IL-2 receptor (IL-2R) Mr 55 kDa The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25 CD122 and CD132. CD25 represents the low affinity chain of the IL-2R a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. 2 CD25 is expressed on activated T cells B cells NK cells and monocytes of all mouse strains tested. 1 3 4 It is transiently expressed at a low level during B-cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. 5 6 CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus. 6 7 The 7D4 monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody 3C7 (Cat. No. 1600-XX). While the monoclonal antibody 7D4 does not block the binding of IL-2 to CD25 when used in combination with the monoclonal antobody 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone. 3 8 9

Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin eyes or clothing. Wear eye or face protection when handling. If skin or eye contact occurs wash with copious amounts of water. If ingested or inhaled contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Membrane; Multi-pass membrane protein.

Strongly down-regulated in the initial phase after sciatic nerve injury.