MOUSE ANTI HUMAN FACTOR VIII

FACTOR VIII

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Recognises human Factor VIII an essential blood coagulation factor. Whilst circulating in the blood it is mostly stably complexed to von Willebrand factor. It is activated through cleavage at various sites dissociates from the complex and interacts with Factor IXa in the presence of calcium ions and phospholipids to convert Factor X to the activated Factor Xa which activates thrombin. Thrombin cleaves fibrinogen into fibrin which polymerises and cross-links to form a blood clot. The activated Factor VIII is proteolytically inactivated and cleared from the bloodstream. Defects in Factor VIII cause haemophilia A a disorder characterised by the body\' s inability to control blood clotting. This could result in severe blood loss even with minor injuries. Clone RFF-VIIIC/8 is a very potent coagulation inhibitor. It recognises an epitope towards the N-terminus of full-length Factor VIII. It also recognises the 210kDa 90kDa and 40kDa cleavage products. This clone does not cross-react with von Willebrand factor. Recommended Secondary Antibodies: Rabbit Anti Mouse IgGGoat Anti Mouse IgGGoat Anti Mouse IgG (H/L)Goat Anti Mouse IgG IgA IgMHuCAL Anti Mouse IgG1Goat Anti Mouse IgG (Fc)Sheep Anti Mouse IgG (H/L) 1. Inohara N. et al (1998) RICK a novel protein kinase containing a caspase recruitment domain interacts with CLARP and regulates CD95-mediated apoptosis. J Biol Chem. 273:12296-300. 2. McCarthy J. V. et al (1998) RIP2 is a novel NF-kappaB-activating and cell death-inducing kinase. J Biol Chem. 273:16968-75. 3. Thome M. et al (1998) Identification of CARDIAK a RIP-like kinase that associates with caspase-1. Curr Biol. 8:885-8. [1] \" RICK a novel protein kinase containing a caspase recruitment domain interacts with CLARP and regulates CD95-mediated apoptosis. \" Inohara N. del Peso L. Koseki T. Chen S. Nunez G. J. Biol. Chem. 273:12296-12300(1998) [PubMed: 9575181] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1) MUTAGENESIS OF LYS-47. [2] \" RIP2 is a novel NF-kappaB-activating and cell death-inducing kinase. \" McCarthy J. V. Ni J. Dixit V. M. J. Biol. Chem. 273:16968-16975(1998) [PubMed: 9642260] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1) MUTAGENESIS OF LYS-47. Tissue: Endothelial cell. [3] \" Identification of CARDIAK a RIP-like kinase that associates with caspase-1. \" Thome M. Hofmann K. Burns K. Martinon F. Bodmer J. -L. Mattmann C. Tschopp J. Curr. Biol. 8:885-888(1998) [PubMed: 9705938] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1) MUTAGENESIS OF ASP-146. Tissue: Pancreatic adenocarcinoma. [4] \" The secreted protein discovery initiative (SPDI) a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. \" Clark H. F. Gurney A. L. Abaya E. Baker K. Baldwin D. T. Brush J. Chen J. Chow B. Chui C. Crowley C. Currell B. Deuel B. Dowd P. Eaton D. Foster J. S. Grimaldi C. Gu Q. Hass P. E. Heldens S. Huang A. Kim H. S. Klimowski L. Jin Y. Johnson S. Lee J. Lewis L. Liao D. Mark M. R. Robbie E. Sanchez C. Schoenfeld J. Seshagiri S. Simmons L. Singh J. Smith V. Stinson J. Vagts A. Vandlen R. L. Watanabe C. Wieand D. Woods K. Xie M. -H. Yansura D. G. Yi S. Yu G. Yuan J. Zhang M. Zhang Z. Goddard A. D. Wood W. I. Godowski P. J. Gray A. M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). [5] \" DNA sequence and analysis of human chromosome 8. \" Nusbaum C. Mikkelsen T. S. Zody M. C. Asakawa S. Taudien S. Garber M. Kodira C. D. Schueler M. G. Shimizu A. Whittaker C. A. Chang J. L. Cuomo C. A. Dewar K. FitzGerald M. G. Yang X. Allen N. R. Anderson S. Asakawa T. Blechschmidt K. Bloom T. Borowsky M. L. Butler J. Cook A. Corum B. DeArellano K. DeCaprio D. Dooley K. T. Dorris L. III Engels R. Gloeckner G. Hafez N. Hagopian D. S. Hall J. L. Ishikawa S. K. Jaffe D. B. Kamat A. Kudoh J. Lehmann R. Lokitsang T. Macdonald P. Major J. E. Matthews C. D. Mauceli E. Menzel U. Mihalev A. H. Minoshima S. Murayama Y. Naylor J. W. Nicol R. Nguyen C. O\' Leary S. B. O\' Neill K. Parker S. C. J. Polley A. Raymond C. K. Reichwald K. Rodriguez J. Sasaki T. Schilhabel M. Siddiqui R. Smith C. L. Sneddon T. P. Talamas J. A. Tenzin P. Topham K. Venkataraman V. Wen G. Yamazaki S. Young S. K. Zeng Q. Zimmer A. R. Rosenthal A. Birren B. W. Platzer M. Shimizu N. Lander E. S. Nature 439:331-335(2006) [PubMed: 16421571] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. [6] \" The status quality and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). \" The MGC Project TeamGenome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Skin. [7] \" Proteomics analysis of protein kinases by target class-selective prefractionation and tandem mass spectrometry. \" Wissing J. Jaensch L. Nimtz M. Dieterich G. Hornberger R. Keri G. Wehland J. Daub H. Mol. Cell. Proteomics 6:537-547(2007) [PubMed: 17192257] [Abstract]Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-531 MASS SPECTROMETRY. Tissue: Leukemic T-cell. [8] \" ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. \" Matsuoka S. Ballif B. A. Smogorzewska A. McDonald E. R. III Hurov K. E. Luo J. Bakalarski C. E. Zhao Z. Solimini N. Lerenthal Y. Shiloh Y. Gygi S. P. Elledge S. J. Science 316:1160-1166(2007) [PubMed: 17525332] [Abstract]Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-176 MASS SPECTROMETRY. Tissue: Embryonic kidney. [9] \" Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. \" Daub H. Olsen J. V. Bairlein M. Gnad F. Oppermann F. S. Korner R. Greff Z. Keri G. Stemmann O. Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-168; SER-176; SER-178; SER-363; SER-527; SER-529; SER-531 AND SER-539 MASS SPECTROMETRY. Tissue: Cervix carcinoma. [10] \" A quantitative atlas of mitotic phosphorylation. \" Dephoure N. Zhou C. Villen J. Beausoleil S. A. Bakalarski C. E. Elledge S. J. Gygi S. P. Proc. Natl. Acad. Sci. U. S. A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-531 MASS SPECTROMETRY. Tissue: Cervix carcinoma.