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Anti-Apolipoprotein A-II (Human)

Anti-Apolipoprotein A-II (Human)

ArtNr	18-732-292279
Hersteller	GENWAY
Menge	1 mg
Kategorie	Metabolism Glucose Homeostasis Cholesterol & Lipid Metabolism Antibodies Obesity Immunohistochemistry Immunoprecipitation Western Blot ELISA Lipid & Fatty Acid Transport Primary Antibodies
Typ	Antibody
Applikationen	WB, IP, IHC, ELISA
Specific against	Human
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	GWB-A197DF
Similar products	18-732-292279
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Genway ID:	GWB-A197DF
Antigen:	apoLipoprotein A2
Immunogen:	apoLipoprotein Type A-II was isolated from human plasma by density gradient centrifugation followed by HPLC purification. Family: ApolipoproteinFraction: Affinity Purified IgGApplication: Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. Recommended
Dilution:	This product was assayed by immunoblot and found to be reactive against apoLipoprotein A-II at a dilution of 1:5 000 to 1:10 000. This product was also assayed against 1. 0 ?g of apoLipoprotein A-II in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H& L] (Rabbit) catalog # 25-732-294061 and (ABTS (2 2\' -azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4 000 to 1:8 000 of the stock concentration is suggested for this product. For immunohistochemistry on paraffin embedded tissue dilute the product 1:50 to 1:200. Optimal titers for other applications should be determined by the researcher. Physical State: Liquid (sterile filtered)Purity and
Specificity:	This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein A-II and has negligible cross-reactivity with Type A-I B C-I C-II C-III E and J apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible. Antibody
Concentration:	1. 0 mg/ml (by UV absorbance at 280 nm)
Buffer:	0. 125M Sodium Borate 0. 075M Sodium Chloride 0. 005M EDTA; pH 8. 0
Stabilizer:	None
Ship Code:	WETICE
Function:	May stabilize HDL (high density lipoprotein) structure by its association with lipids and affect the HDL metabolism.
Subunit:	Homodimer; disulfide-linked. Also forms a disulfide-linked heterodimer with APOD. Interacts with HCV core protein. Interacts with APOA1BP.
Subcellular Location:	Secreted.
Tissue Specificity:	Plasma; synthesized in the liver and intestine. Mass Spectrometry: Mass=8701. 2; Method=MALDI; Range=24-100; Source=PubMed:12562854; . Mass Spectrometry: Mass=8823. 4; Method=MALDI; Range=24-100; Note=Cysteinylated ApoA-II; Source=PubMed:12562854; . Mass Spectrometry: Mass=17421. 3; Method=MALDI; Range=24-100; Note=Homodimer; Source=PubMed:12562854; . Mass Spectrometry: Mass=17293. 4; Method=MALDI; Range=24-100; Note=Heterodimer with apolipoprotein A-II(1-76); Source=PubMed:12562854; . Mass Spectrometry: Mass=8578. 3; Method=MALDI; Range=24-99; Source=PubMed:12562854; . Mass Spectrometry: Mass=17166. 2; Method=MALDI; Range=24-99; Note=Homodimer; Source=PubMed:12562854; .
Similarity:	Belongs to the apolipoprotein A2 family.

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Menge: 1 mg

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