M-MuLV Reverse Transcriptase

GenScript M-MuLV Reverse Transcriptase (M-MLV) synthesizes a complementary cDNA strand initiating from a primer using RNA as a template (cDNA synthesis), making it ideal for a wide range of applications. M-MMLV is derived from a cloned region of the pol gene of M-MLV and isolated from an E. coli strain overexpressing this construct. To increase cDNA yields and get a higher percentage of longer transcripts, the M-MLV Reverse Transcriptase has been modified with reduced RNase H activity, and expressed free of exogenous RNases and other nucleases. One unit is defined as the amount of enzyme required to catalyze the transfer of 1 nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37C.

PCR & Cloning