ArtNr |
Z02974-10 |
Hersteller |
GenScript
|
Menge |
10 ug |
Kategorie |
|
Typ |
Proteins |
Specific against |
Human |
Purity |
> 95% by SDS-PAGE and HPLC analyses. |
Sequence |
Thr27-Pro200 (accession number:Q8N4W3), expressed with an N-terminal Met. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
protein/Z02974_10-G_CSF_Human,Granulocyte Colony-Stimulating Factor (G-CSF), contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17. Recombinant, human Granulocyte Colony-Stimulating Factor (rhG-CSF), produced in E. coliis a single non-glycosylated polypeptide chain containing 175amino acids. A fully biologically active molecule, rhG-CSF is obtained by proprietary chromatographic techniques at GenScript, with an apparent molecular mass of 18.8kDa analyzed by reducing SDS-PAGE.</td></tr><tr><th>M.W.</th><td colspan="7"> 18.8kDa, observed by reducing SDS-PAGE.</td></tr><tr><th>Purity</th><td colspan="7"> > 95% by SDS-PAGE and HPLC analyses.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> <0.2 EU/ug, determined by LAL method.</td></tr><tr><th>Specific Activity</th><td colspan="7"> ED50, 1.0× 107 IU/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized recombinant, human Granulocyte Colony-Stimulating Factor (rhG-CSF), remains stable up to 6 months at -80C from date of receipt. Upon reconstitution, rhG-CSF should be stable up to 2 weeks at 4C or up to 3 months at -20C.</td></tr><tr><th>Formulation</th><td colspan="7"> Lyophilized after extensive dialysis against 25mM Tris, pH8.0.</td></tr><tr><th>Reconstitution</th><td colspan="7"> Reconstituted in ddH2O at 100 ug/ml.</td></tr><tr><th>Sequence</th><td colspan="7"> Thr27-Pro200 (accession number:Q8N4W3), expressed with an N-terminal Met.</td></tr><tr><th>Note</th><td colspan="7"> For research use only</td></tr> |
Similar products |
G-CSF |
Lieferbar |
|
Specificity |
ED50, 1.0x 107 IU/mg. |
Specificity |
ED50, 1.0x 107 IU/mg. |
Description |
Granulocyte Colony-Stimulating Factor (G-CSF), contains internal disulfide bonds. Among the family of colony-stimulating factors, Granulocyte Colony Stimulating Factor (G-CSF) is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of Granulocyte Colony Stimulating Factor (G-CSF) can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of Granulocyte Colony Stimulating Factor (G-CSF). In epithelial, endothelial, and fibroblastic cells secretion of Granulocyte Colony Stimulating Factor (G-CSF) is induced by Interleukin-17. Recombinant, human Granulocyte Colony-Stimulating Factor (rhG-CSF), produced in E. coliis a single non-glycosylated polypeptide chain containing 175amino acids. A fully biologically active molecule, rhG-CSF is obtained by proprietary chromatographic techniques at GenScript, with an apparent molecular mass of 18.8kDa analyzed by reducing SDS-PAGE. |
Endotoxin Level |
<0.2 EU/ug, determined by LAL method. |
Formulation |
Lyophilized after extensive dialysis against 25mM Tris, pH8.0. |
M.W. |
18.8kDa, observed by reducing SDS-PAGE. |
Note |
For research use only |
Product Line |
Cytokine, Chemokines & Growth Factors |
Reconstitution |
Reconstituted in ddH2O at 100 ug/ml. |
Storage |
Lyophilized recombinant, human Granulocyte Colony-Stimulating Factor (rhG-CSF), remains stable up to 6 months at -80C from date of receipt. Upon reconstitution, rhG-CSF should be stable up to 2 weeks at 4C or up to 3 months at -20C. |
Manufacturers Category |
Proteins |
Manufacturers Product Line |
Growth Factors |
Product Origin |
USA |
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