ArtNr |
Z02021-10 |
Hersteller |
GenScript
|
Menge |
10 ug |
Kategorie |
|
Typ |
Proteins |
Specific against |
Human |
Purity |
Greater than 98.0% as determined by: 1. Analysis by RP-HPLC 2. Anion-exchange FPLC 3. Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel |
Citations |
Gessi M, et al . Parathyroid hormone-related peptide (hPTHrP) and parathyroid hormone-related peptide receptor type 1 (PTHR1) expression in human thymus. J. Histochem. Cytochem. Aug 2005, 53(8): 955-962. Huan J, et al . Parathyroid hormone 7-84 induces hypocalcemia and inhibits the parathyroid hormone 1-84 secretory response to hypocalcemia in rats with intact parathyroid glands. J. Am. Soc. Nephrol. Jul 2006, 17(7): 1923-1930. Mahon MJ, Segre GV. Stimulation by parathyroid hormone of a NHERF-1-assembled complex consisting of the parathyroid hormone I receptor, phospholipase Cbeta, and actin increases intracellular calcium in opossum kidney cells. J. Biol. Chem. May 2004, 279(22): 23550-23558. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
protein/Z02021_10-Parathyroid_Hormone_PTH_1_84_Human,Parathyroid Hormone (PTH), is the most important endocrine regulator of calcium and phosphorus concentration in extracellular fluid. Parathyroid Hormone (PTH) is secreted from cells of the parathyroid glands and finds Parathyroid Hormone's major target cells in bone and kidney. Like most other protein hormones, Parathyroid Hormone (PTH) is synthesized as a preprohormone. After intracellular processing, the mature hormone is packaged within the Golgi into secretory vesicles, the secreted into blood by exocytosis. Parathyroid Hormone (PTH) is secreted as a linear protein of 84 amino acids. Recombinant, Human Parathyroid Hormone (PTH), produced in, E. coli, is a single, non-glycosylated, polypeptide chain containing 84 amino acids and having a molecular mass of 9,000 Da.</td></tr><tr><th>M.W.</th><td colspan="7"> 9,000 Da</td></tr><tr><th>Purity</th><td colspan="7"> Greater than 98.0% as determined by: 1. Analysis by RP-HPLC 2. Anion-exchange FPLC 3. Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1 ng/ug (1 EU/ug) of Human Parathyroid Hormone (PTH) .</td></tr><tr><th>Specific Activity</th><td colspan="7"> PTH is fully biologically active when compared to standards. The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 1.0×104 U/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized, Human Parathyroid Hormone (PTH ), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution Human Parathyroid Hormone (PTH )should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles.</td></tr><tr><th>Formulation</th><td colspan="7"> The protein (1 mg/ml) was lyophilized after extensive dialyses against 1.15 mg sodium citrate, sodium chloride 7.31 mg, 0.21 mg citric acid, 0.1117 mg EDTA-Na2, 0.2 mg Tween 80 and 50 mg Mannitol.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended to reconstitute the lyophilized PTH in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.</td></tr><tr><th>Reference</th><td colspan="7">Gessi M, et al . Parathyroid hormone-related peptide (hPTHrP) and parathyroid hormone-related peptide receptor type 1 (PTHR1) expression in human thymus. J. Histochem. Cytochem. Aug 2005, 53(8): 955-962. Huan J, et al . Parathyroid hormone 7-84 induces hypocalcemia and inhibits the parathyroid hormone 1-84 secretory response to hypocalcemia in rats with intact parathyroid glands. J. Am. Soc. Nephrol. Jul 2006, 17(7): 1923-1930. Mahon MJ, Segre GV. Stimulation by parathyroid hormone of a NHERF-1-assembled complex consisting of the parathyroid hormone I receptor, phospholipase Cbeta, and actin increases intracellular calcium in opossum kidney cells. J. Biol. Chem. May 2004, 279(22): 23550-23558.</td></tr> |
Similar products |
Parathyroid |
Lieferbar |
|
Specificity |
PTH is fully biologically active when compared to standards. The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 1.0x104 U/mg. |
Specificity |
PTH is fully biologically active when compared to standards. The activity calculated by UMR106 cell/cAMP method corresponding to a specific activity of 1.0x104 U/mg. |
Description |
Parathyroid Hormone (PTH), is the most important endocrine regulator of calcium and phosphorus concentration in extracellular fluid. Parathyroid Hormone (PTH) is secreted from cells of the parathyroid glands and finds Parathyroid Hormone's major target cells in bone and kidney. Like most other protein hormones, Parathyroid Hormone (PTH) is synthesized as a preprohormone. After intracellular processing, the mature hormone is packaged within the Golgi into secretory vesicles, the secreted into blood by exocytosis. Parathyroid Hormone (PTH) is secreted as a linear protein of 84 amino acids. Recombinant, Human Parathyroid Hormone (PTH), produced in, E. coli, is a single, non-glycosylated, polypeptide chain containing 84 amino acids and having a molecular mass of 9, 000 Da. |
Endotoxin Level |
Less than 0.1 ng/ug (1 EU/ug) of Human Parathyroid Hormone (PTH) . |
Formulation |
The protein (1 mg/ml) was lyophilized after extensive dialyses against 1.15 mg sodium citrate, sodium chloride 7.31 mg, 0.21 mg citric acid, 0.1117 mg EDTA-Na2, 0.2 mg Tween 80 and 50 mg Mannitol. |
M.W. |
9, 000 Da |
Product Line |
Cytokine, Chemokines & Growth Factors |
Reconstitution |
It is recommended to reconstitute the lyophilized PTH in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
Storage |
Lyophilized, Human Parathyroid Hormone (PTH ), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution Human Parathyroid Hormone (PTH )should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles. |
Manufacturers Category |
Proteins |
Manufacturers Product Line |
Hormones |
Product Origin |
USA |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.