ArtNr |
Z00372-1 |
Hersteller |
GenScript
|
Menge |
1mg(2x500ug) |
Kategorie |
|
Typ |
Proteins |
Specific against |
Human |
Purity |
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC (b) Anion-exchange FPLC (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel |
Sequence |
PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM |
Citations |
Wernstedt I, et al . Reduced stress- and cold-induced increase in energy expenditure in IL-6 deficient mice. Am. J. Physiol. Regul. Integr. Comp. Physiol.Feb 2006, Itoh S, et al . A critical role for interleukin-6 family-mediated Stat3 activation in osteoblast differentiation and bone formation. Bone. Sep 2006, 39 (3): 505-512. Pang Y, et al . Role of interleukin-6 in lipopolysaccharide-induced brain injury and behavioral dysfunction in neonatal rats. Neuroscience. Aug 2006, 141 (2): 745-755. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
protein/Z00372_1-Interleukin_6_IL_6_Human,Interleukin-6 (IL-6), is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. Interleukin-6 (IL-6) induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor, interferon-beta-2, monocyte derived human B cell growth factor, B cell stimulating factor, hepatocyte stimulating factor, Interleukin Hybridoma/Plasmacytoma-1. Recombinant human Interleukin-6 (IL-6), that is produced in, E. coli, and purified by proprietary chromatographic techniques is a single, non-glycosylated polypeptide chain containing 185 amino acids and having a molecular mass of 21,000 Da.</td></tr><tr><th>M.W.</th><td colspan="7"> 21,000 Da</td></tr><tr><th>Purity</th><td colspan="7"> Greater than 98.0% as determined by: (a) Analysis by RP-HPLC (b) Anion-exchange FPLC (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1ng/ug (1 EU/ug) determined by LAL test.</td></tr><tr><th>Specific Activity</th><td colspan="7"> The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 5×107 IU/mg. </td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized, recombinant human Interleukin-6 (IL-6), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution recombinant human Interleukin-6 (IL-6) should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.</td></tr><tr><th>Formulation</th><td colspan="7"> The protein was lyophilized after extensive dialysis against 20mM HAc-NaAc, pH5.0, 150mM NaAc buffer.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended to reconstitute the lyophilized recombinant human Interleukin-6 (IL-6) in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.</td></tr><tr><th>Sequence</th><td colspan="7"> PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM </td></tr><tr><th>Reference</th><td colspan="7">Wernstedt I, et al . Reduced stress- and cold-induced increase in energy expenditure in IL-6 deficient mice. Am. J. Physiol. Regul. Integr. Comp. Physiol.Feb 2006, Itoh S, et al . A critical role for interleukin-6 family-mediated Stat3 activation in osteoblast differentiation and bone formation. Bone. Sep 2006, 39 (3): 505-512. Pang Y, et al . Role of interleukin-6 in lipopolysaccharide-induced brain injury and behavioral dysfunction in neonatal rats. Neuroscience. Aug 2006, 141 (2): 745-755.</td></tr> |
Similar products |
Interleukin-6 |
Lieferbar |
|
Specificity |
The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 5x107 IU/mg. |
Specificity |
The ED50 as determined by the dose-dependant stimulation of murine 7TD1 cells is < 0.1 ng/ml, corresponding to a Specific Activity of 5x107 IU/mg. |
Description |
Interleukin-6 (IL-6), is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. Interleukin-6 (IL-6) induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor, interferon-beta-2, monocyte derived human B cell growth factor, B cell stimulating factor, hepatocyte stimulating factor, Interleukin Hybridoma/Plasmacytoma-1. Recombinant human Interleukin-6 (IL-6), that is produced in, E. coli, and purified by proprietary chromatographic techniques is a single, non-glycosylated polypeptide chain containing 185 amino acids and having a molecular mass of 21, 000 Da. |
Endotoxin Level |
Less than 0.1ng/ug (1 EU/ug) determined by LAL test. |
Formulation |
The protein was lyophilized after extensive dialysis against 20mM HAc-NaAc, pH5.0, 150mM NaAc buffer. |
M.W. |
21, 000 Da |
Product Line |
Cytokine, Chemokines & Growth Factors |
Reconstitution |
It is recommended to reconstitute the lyophilized recombinant human Interleukin-6 (IL-6) in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. |
Storage |
Lyophilized, recombinant human Interleukin-6 (IL-6), remains stable at room temperature for three weeks, but it is best stored desiccated below -18C. Upon reconstitution recombinant human Interleukin-6 (IL-6) should be stored at 4C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles. |
Product Origin |
USA |
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