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IL-1RA, Human Europäischer Partner

ArtNr Z00367-10
Hersteller GenScript
Menge 10 ug
Kategorie
Typ Proteins
Specific against Human
Purity Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Citations Dayer JM, et al . Adipose Tissue Has Anti-Inflammatory Properties: Focus on IL-1 Receptor Antagonist (IL-1Ra). Ann. N. Y. Acad. Sci. Jun 2006, 1069: 444-453. Bessler H, et al . Postoperative pain, morphine consumption, and genetic polymorphism of IL-1beta and IL-1 receptor antagonist. Neurosci. Lett. Aug 2006, 404(1-2): 154-158.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias protein/Z00367_10-Interleukin_1_Receptor_Antagonist_IL_1ra_Human,Interleukin-1 receptor antagonist (IL-1ra), is a member of the IL-1 family. Endogenous IL-1ra is produced in numerous animal disease models as well as in human autoimmune and chronic inflammatory diseases. It binds to IL-1 receptors in competition with IL-1, but does not elicit intracellular response from this binding. Its role in counteracting the proinflammatory effects of IL-1 is being studied by numerous research groups. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL1-ra. The regulated expression of IL1ra in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1ra is markedly enhanced by IL-1 , TNF-alpha , or PDGF. Recombinant Human IL-1RN produced in, E. coli, is a non-glycosylated, N-terminal methionyl form of the human naturally-occurring polypeptide chain containing 153 amino acids and having a molecular mass of 17,258 Da.</td></tr><tr><th>M.W.</th><td colspan="7"> 17,258 Da</td></tr><tr><th>Purity</th><td colspan="7"> Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1 ng/ug (IEU/ug) of IL-1 ra.</td></tr><tr><th>Specific Activity</th><td colspan="7"> The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml, corresponding to a Specific Activity of 2.0×106 IU/mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized rHuIL-1 ra although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution rHuIL-1ra should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.</td></tr><tr><th>Formulation</th><td colspan="7"> The protein was lyophilized after extensive dialysis against 50mM Tris-HCl, pH7.5, 200mM NaCl buffer.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended to reconstitute the lyophilized rHuIL-1ra in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. </td></tr><tr><th>Reference</th><td colspan="7">Dayer JM, et al . Adipose Tissue Has Anti-Inflammatory Properties: Focus on IL-1 Receptor Antagonist (IL-1Ra). Ann. N. Y. Acad. Sci. Jun 2006, 1069: 444-453. Bessler H, et al . Postoperative pain, morphine consumption, and genetic polymorphism of IL-1beta and IL-1 receptor antagonist. Neurosci. Lett. Aug 2006, 404(1-2): 154-158.</td></tr>
Similar products Interleukin-1
Lieferbar
Specificity The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml, corresponding to a Specific Activity of 2.0x106 IU/mg.
Specificity
The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml, corresponding to a Specific Activity of 2.0x106 IU/mg.
Description
Interleukin-1 receptor antagonist (IL-1ra), is a member of the IL-1 family. Endogenous IL-1ra is produced in numerous animal disease models as well as in human autoimmune and chronic inflammatory diseases. It binds to IL-1 receptors in competition with IL-1, but does not elicit intracellular response from this binding. Its role in counteracting the proinflammatory effects of IL-1 is being studied by numerous research groups. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL1-ra. The regulated expression of IL1ra in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1ra is markedly enhanced by IL-1 , TNF-alpha , or PDGF. Recombinant Human IL-1RN produced in, E. coli, is a non-glycosylated, N-terminal methionyl form of the human naturally-occurring polypeptide chain containing 153 amino acids and having a molecular mass of 17, 258 Da.
Endotoxin Level
Less than 0.1 ng/ug (IEU/ug) of IL-1 ra.
Formulation
The protein was lyophilized after extensive dialysis against 50mM Tris-HCl, pH7.5, 200mM NaCl buffer.
M.W.
17, 258 Da
Product Line
Cytokine, Chemokines & Growth Factors
Reconstitution
It is recommended to reconstitute the lyophilized rHuIL-1ra in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Storage
Lyophilized rHuIL-1 ra although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution rHuIL-1ra should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles.
Manufacturers Category
Proteins
Manufacturers Product Line
Cytokines
Product Origin
USA

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 10 ug
Lieferbar: In stock
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