EGF, Human

ArtNr	Z00333-10
Hersteller	GenScript
Menge	10 ug
Kategorie	Oncology Food Safety Neuroscience Metabolism Glucose Homeostasis Neural Signaling By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines Adenocarcinoma Contaminants Astrocytoma Metals By Organ Lung Cancer Bladder Cancer
Typ	Proteins
Specific against	Human (Homo sapiens)
Purity	Greater than 95% as determined by, (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Sequence	NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Citations	Katagiri S, Takahashi Y. Potential relationship between normalization of endometrial epidermal growth factor profile and restoration of fertility in repeat breeder cows. Anim. Reprod. Sci. Sep 2006, 95(1-2): 54-66. Endo K, et al . Evaluation of the epidermal growth factor receptor gene mutation and copy number in non-small cell lung cancer with gefitinib therapy. Oncol. Rep. Sep 2006, 16(3): 533-541.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	protein/Z00333-Epidermal_Growth_Factor_EGF_human,Epidermal Growth Factor (EGF), is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant human Epidermal Growth Factor (EGF), is a 6,200 Da protein containing 53 amino acid residues.</td></tr><tr><th>M.W.</th><td colspan="7"> 6.0 kDa+/-10% determined by reduced SDS-PAGE</td></tr><tr><th>Purity</th><td colspan="7"> Greater than 95% as determined by, (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel</td></tr><tr><th>Endotoxin Level</th><td colspan="7"> Less than 0.1ng/ug (1 EU/ug) of recombinant human Epidermal Growth Factor (EGF) as determined by LAL test.</td></tr><tr><th>Specific Activity</th><td colspan="7"> The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0×105 IU/ mg.</td></tr><tr><th>Storage</th><td colspan="7"> Lyophilized recombinant, Human Epidermal Growth Factor (rhEGF), remains stable up to 12 months at -80C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4C or up to 6 months at -20C.</td></tr><tr><th>Formulation</th><td colspan="7">Recombinant human Epidermal Growth Factor (EGF), was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 200mM NaCl buffer.</td></tr><tr><th>Reconstitution</th><td colspan="7"> It is recommended to reconstitute the lyophilized, recombinant human Epidermal Growth Factor (EGF), in sterile 18 M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.</td></tr><tr><th>Sequence</th><td colspan="7"> NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR</td></tr><tr><th>Reference</th><td colspan="7">Katagiri S, Takahashi Y. Potential relationship between normalization of endometrial epidermal growth factor profile and restoration of fertility in repeat breeder cows. Anim. Reprod. Sci. Sep 2006, 95(1-2): 54-66. Endo K, et al . Evaluation of the epidermal growth factor receptor gene mutation and copy number in non-small cell lung cancer with gefitinib therapy. Oncol. Rep. Sep 2006, 16(3): 533-541.</td></tr>
Similar products	Epidermal
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Specificity	The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0x105 IU/ mg.
Specificity	The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0x105 IU/ mg.
Description	Epidermal Growth Factor (EGF), is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant human Epidermal Growth Factor (EGF), is a 6, 200 Da protein containing 53 amino acid residues.
Endotoxin Level	Less than 0.1ng/ug (1 EU/ug) of recombinant human Epidermal Growth Factor (EGF) as determined by LAL test.
Formulation	Recombinant human Epidermal Growth Factor (EGF), was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 200mM NaCl buffer.
M.W.	6.0 kDa+/-10% determined by reduced SDS-PAGE
Product Line	Cytokine, Chemokines & Growth Factors
Reconstitution	It is recommended to reconstitute the lyophilized, recombinant human Epidermal Growth Factor (EGF), in sterile 18 M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Storage	Lyophilized recombinant, Human Epidermal Growth Factor (rhEGF), remains stable up to 12 months at -80C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4C or up to 6 months at -20C.
Manufacturers Category	Proteins
Manufacturers Product Line	Growth Factors
Product Origin	USA

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