Application info |
Working concentrations for specific applications should be determined by the investigators. The appropriate concentrations may be affected by primary/secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Western Blot: 1:1, 000-1:5, 000 ELISA: 1:5, 000-1:10, 000 Other applications: user-optimized Optimal working dilutions must be determined by the end user. |
Description |
A camelid single-domain antibody (sdAb, called Nanobody by Ablynx, the developer) is a peptide chain of about 110 amino acids long, comprising one variable domain of a heavy-chain antibody, these are called VHH fragments. Like a whole antibody, it is able to bind selectively to a specific antigen. Single-domain antibodies allow a broad range of applications in biotechnical as well as therapeutic uses due to their small size, simple production and high affinity. GenScript MonoRab Rabbit Anti-Camelid VHH Cocktail is a unique secondary antibody, composed of several rabbit anti-Camelid VHH monoclonal antibodies. Those antibodies were produced from hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a rabbit immunized with llama VHH antibody. |