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Alpha-Synuclein (alpha-Synuclein, Alpha Synuclein, SNCA; Human) High Sensitivity ELISA Assay Kit

Hersteller Fivephoton
Kategorie
Typ Elisa-Kit
Specific against Human, Mouse, Rat, Canine
Menge 1 Kit, 96T
ArtNr hSNCA-ELISA
Targets SNCA
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Poisonous Material
Sodium Azide (0.1%)
Highlights
Fully pre-configured sandwich ELISA format providing technical convenience and high sensitivity
Strip plate cassette configuration allowing separate use and storage of ELISA plate sections
Detection Range: 3.0-135 pg/mL
Standard Peptide Concentration: 135 pg/mL
Affinity purified polyclonal capture antibody coated on plate. Separate HRP-conjugated detection antiobody
Rapid colorimetric detection. 1 hr total incubation at 37C; 2 hr incubation at RT
Background
Alpha-Synuclein: Alpha-synuclein (SNCA gene) is a 140 amino acid protein expressed in brain that is associated with fibrillar structures in Lewy Bodies. Although primary located in the cytosol, alpha-synuclein binds to phospholipid and may assemble into pore-like structures in cellular membranes. Generation of aggregates in Lewy Bodies and/or association of mutant or wild-type alpha-synuclein with membraneous structures have been attributed to the etiology of both early-onset congenital and age-related Parkinson’s Disease.

Parkinson's disease is a complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. Additional features of the disease include the characteristic postural abnormalities, dysautonomia, dystonic cramps, and dementia. The pathology of Parkinson disease involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies in brain and spinal cord.
References
Adamczyk, A. et al. (2007) Alpha-synuclein decreases arachidonic acid incorporation into rat striatal synaptoneurosomes. Folia Neuropathol 45 (4), 230-235

Kim, H.J. et al. (2007) Seed-dependent accelerated fibrillation of alpha-synuclein induced by periodic ultrasonication treatment. J Microbiol Biotechnol 17 (12), 2027-2032

Wakabayashi, K. et al. (2007) The Lewy body in Parkinson's disease: molecules implicated in the formation and degradation of alpha-synuclein aggregates. Neuropathology 27 (5), 494-506

Yoshida, M. (2007) Multiple system atrophy: alpha-synuclein and neuronal degeneration. Neuropathology 27 (5), 484-493

Kloepper, K.D. et al. (2007) Solid-state NMR spectroscopy reveals that water is nonessential to the core structure of alpha-synuclein fibrils. J Phys Chem B 111 (47), 13353-13356

Buchman, V.L. and Ninkina, N. (2008) Modulation of alpha-synuclein expression in transgenic animals for modelling synucleinopathies--is the juice worth the squeeze? Neurotox Res 14 (4), 329-341

Fuchs, J. et al. (2008) Genetic variability in the SNCA gene influences alpha-synuclein levels in the blood and brain. Faseb J 22 (5), 1327-1334

Parihar, M.S. et al. (2009) Alpha-synuclein overexpression and aggregation exacerbates impairment of mitochondrial functions by augmenting oxidative stress in human neuroblastoma cells. Int J Biochem Cell Biol 41 (10), 2015-2024

Gibrat, C. et al. (2009) Differences between subacute and chronic MPTP mice models: investigation of dopaminergic neuronal degeneration and alpha-synuclein inclusions. J Neurochem 109 (5), 1469-1482

Kim, S. et al. (2009) Alpha-synuclein induces migration of BV-2 microglial cells by up-regulation of CD44 and MT1-MMP. J Neurochem 109 (5), 1483-1496

Leong, S.L. et al. (2009) Modulation of alpha-synuclein aggregation by dopamine: a review. Neurochem Res 34 (10), 1838-1846

Xilouri, M. et al. (2009) Abberant alpha-synuclein confers toxicity to neurons in part through inhibition of chaperone-mediated autophagy. PLoS One 4 (5), e5515

Noguchi-Shinohara, M. et al. (2009) CSF alpha-synuclein levels in dementia with Lewy bodies and Alzheimer's disease. Brain Res 1251, 1-6

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 Kit, 96T
Lieferbar: In stock
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