Highlights |
Strong cell lysis and protein extraction capacity while preserving protein conformation and protein-protein interaction for co-immunoprecipitation (Co-IP) analysis. Ingredients: 0.5% CHAPS, HEPES, NaCl Effective as a stand alone reagent for cell lysis and protein extraction as well as for immunoprecipitation. Contains a non-amine buffer (HEPES) enabling cross-linking with NHS-ester derivatives. Compatible with Bradford, Lowry and BCA protein assays. 60 ml volume: Suitable for two hundred 10-cm culture plates, or six hundred wells in 6-well dishes. 120 ml volume is also available. Storage: 4C. Ships at ambient temperature. |
References |
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Farmery MR, Allen S, Allen AJ, Bulleid NJ. 2000. The role of ERp57 in disulfide bond formation during the assembly of major histocompatibility complex class I in a synchronized semipermeabilized cell translation system. J Biol Chem 275: 14933-8.
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Pieren M, Gali C, et. al. 2005. The use of calnexin and calreticulin in cellular and viral glycoproteins. J Biol Chem 280: 28265-71.
Zhu X, Peng J, Chen D, Liu X, Ye L, et al. 2005. Calnexin and ERp57 facilitate the assembly of the neonatal Fc. Journal of Immunology 175: 967-76. |