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FD NeuroApop™ Kit

FD NeuroApop™ Kit

ArtNr	PK201
Hersteller	FD NEUROTECHNOLOGIES
Menge	1 kit
Kategorie	Neuroscience Other Assays & Kits Other Kits
Typ	Kit
Specific against	other
Citations	Gavrieli Y, Sherman Y, and Ben-Sasson SA. (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:493-501. Wyllie AH. (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature. 284:555-6. Arends MJ, Morris RG, and Wyllie AH. (1990) Apoptosis: the role of the endonuclease. Amer. J. Pathol. 136:593-608. Hsu SM, Raine L, and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29:577-80.
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Lieferbar
Manufacturer - Category	Specialties and Accessories
Description	FD NeuroApop™ Kit is specifically designed for the detection of neuronal apoptosis in tissue sections from the central nervous system based on the principle of in situ DNA nick-end labeling (TUNEL) technique¹ . The assay uses terminal deoxynucleotidyl transferase to catalyze the incorporation of biotinylated deoxyuridines onto the free 3'-hydroxyl termini of DNA fragments, which are considered one of the most characteristic features of apoptosis^{2, 3}. The integrated biotins are amplified and visualized with the avidin-biotin-complex (ABC) method⁴, enabling light microscopic identification. The reagents and procedure of FD NeuroApop™ Kit have been optimized to achieve a high degree of both specificity and sensitivity for detecting apoptotic neurons with the lowest background. This kit can be used with frozenand paraffin sections, as well as cultured cells (cf. photo samples below). The procedure of the kit takes approximately 4 hours.
Materials required but not included	Double distilled water Humidified chamber Incubator or waterbath (30° C) Histological supplies and equipment, including microscope slides, glass coverslips, staining jars, fine-tipped forceps, ethanol, xylenes or xylene-substitutes, mounting medium, and a light microscope.
Part I (Store at -20° C)	Digestive Enzyme 2ml x 4 Reaction Solution A 2ml x 2 Reaction Solution B 85µ l Reaction Solution C 60µ l Chromogen Solution 20 ml
Part II (Store at 4° C)	Equilibration Buffer 20ml Detection Reagent 5ml 10x Phosphate-Buffered Saline 250ml x 2

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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PK201-datasheet.pdf

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