ArtNr |
SEG685Hu-5x96T |
Hersteller |
Cloud-Clone
|
Menge |
5x96T |
Kategorie |
|
Typ |
Elisa-Kit |
Specific against |
Human |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Alias |
MYOP, Sarcomeric Protein Myopalladin,145 kDa |
Lieferbar |
|
Quantity options |
|
Detection range |
0.156-10ng/mL |
Organism species |
Homo sapiens (Human) |
Sensitivity |
The minimum detectable dose of this kit is typically less than 0.066ng/mL |
Sample type |
Tissue homogenates and other biological fluids. |
Assay length |
3h |
Method |
Double-antibody Sandwich |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Myopalladin (MYPN). No significant cross-reactivity or interference between Myopalladin (MYPN) and analogues was observed. |
Precision |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myopalladin (MYPN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myopalladin (MYPN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
Assay procedure summary |
1. Prepare all reagents, samples and standards; 2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C; 8. Add 50µ L Stop Solution. Read at 450nm immediately. |
Test principle |
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myopalladin (MYPN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myopalladin (MYPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myopalladin (MYPN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myopalladin (MYPN) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
Research Area |
Cardiovascular biology; |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.